Relationship between susceptibility of Blackface sheep to Teladorsagia circumcincta infection and an inflammatory mucosal T cell response
| Autor: | Anton G Gossner, Virginia M Venturina, Darren J Shaw, Josephine M Pemberton, John Hopkins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
T cell T cells Sheep Diseases Real-Time Polymerase Chain Reaction T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Ostertagiasis Species Specificity parasitic diseases medicine Helminths Animals Lymph node Feces 030304 developmental biology Nematode 0303 health sciences lcsh:Veterinary medicine Sheep General Veterinary biology Research Ostertagia Teladorsagia circumcincta veterinary(all) medicine.anatomical_structure Gene Expression Regulation Larva Immunology biology.protein lcsh:SF600-1100 Cytokines Th17 Cells Histopathology Lymph Disease Susceptibility Antibody Breeding values 030215 immunology |
| Zdroj: | Gossner, A G, Venturina, V M, Shaw, D J, Pemberton, J M & Hopkins, J 2012, ' Relationship between susceptibility of Blackface sheep to Teladorsagia circumcincta infection and an inflammatory mucosal T cell response ', Veterinary Research, vol. 43, no. 1, 26, pp.-. https://doi.org/10.1186/1297-9716-43-26 Veterinary Research Veterinary Research, Vol 43, Iss 1, p 26 (2012) |
| DOI: | 10.1186/1297-9716-43-26 |
| Popis: | Teladorsagia circumcincta is the most economically important gastrointestinal (abomasal) nematode parasite of sheep in cool temperate regions, to which sheep show genetically-varying resistance to infection. Lambs, from parents with genetic variation for resistance, were trickle infected with L3 larvae over 12 weeks. 45 lambs were identified with a range of susceptibilities as assessed by: adult worm count at post mortem, faecal egg count (FEC) and IgA antibody levels. This project investigated the correlation of T cell cytokine expression and resistance to infection at the mature stage of response, when the resistant lambs had excluded all parasites. Histopathology showed only minor changes in resistant animals with a low level lymphocyte infiltration; but in susceptible lambs, major pathological changes were associated with extensive infiltration of lymphocytes, eosinophils and neutrophils. Absolute quantitative RT-qPCR assays on the abomasal lymph node (ALN) revealed a significant positive correlation between IL6, IL21 and IL23A transcript levels with adult worm count and FEC. IL23A was also negatively correlated with IgA antibody levels. Significantly positive correlation of TGFB1 levels with adult worm count and FEC were also seen in the abomasal mucosa. These data are consistent with the hypothesis that the inability to control L3 larval colonization, adult worm infection and egg production is due to the activation of the inflammatory Th17 T cell subset. |
| Databáze: | OpenAIRE |
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