Humoral immunity in malignant skin melanoma. Isolation of melanoma specific IgG from melanoma metastases
| Autor: | E. Kristensen, R. Reimann, E. Langvad |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Skin Neoplasms
Antibodies Neoplasm Cell chemistry.chemical_compound Antigen Antibody Specificity medicine Humans Neoplasm Metastasis Melanoma biology General Medicine Complement fixation test medicine.disease Immunoglobulin A Papain medicine.anatomical_structure chemistry Immunoglobulin G Humoral immunity Immunology Antibody Formation biology.protein Antibody Skin melanoma |
| Zdroj: | European journal of cancer. 12(12) |
| ISSN: | 0014-2964 |
| Popis: | Three M potassium chloride extracts of malignant melanoma, hypernephroma and colonic carcinoma were found to consume complement in the absence of patient sera. Treatment of the tumour extracts with papain abolished the anti-complementary effect. IgG and IgA present in tumour extracts were identified in cross-over electrophoresis by means of monospecific antibodies. Purified melanoma-derived immunoglobulins were reacted in complement fixation test against the 3 M KCl extract of an established melanoma cell line with positive result. The presence of specific antimelanoma IgG as well as native common melanoma antigen was thus established. It is concluded that specific antimelanoma antibodies are present within the clinical tumour whereas the actual localization of these antibodies, i.e., whether present on tumour cell membranes or on infiltrating lymphocytes, remains an open question. Sera from 93 patients suffering from stage I and II skin melanoma were assayed for circulating antibodies against antigen present in the extract of an established melanoma cell line. A positive CFT was established in 27% of the patients. The discrepancy between this finding and that of other investigators evades good explanation. |
| Databáze: | OpenAIRE |
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