Morphology of temperate bacteriophage SfV and characterisation of the DNA packaging and capsid genes: the structural genes evolved from two different phage families
| Autor: | Dario C Angeles, Pham Thi Huan, Gwen E. Allison, Naresh K. Verma |
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| Rok vydání: | 2003 |
| Předmět: |
Genes
Viral Sequence analysis viruses Molecular Sequence Data Myoviridae Siphoviridae Shigella flexneri Bacteriophage Open Reading Frames Capsid Species Specificity Virology Endopeptidases Bacteriophages Temperate bacteriophage Amino Acid Sequence Antigens Viral Peptide sequence Genetics Endodeoxyribonucleases biology Structural gene Nucleic acid sequence biochemical phenomena metabolism and nutrition biology.organism_classification Protease Molecular Weight DNA Viral Capsid Proteins Terminase Sequence Alignment Sequence Analysis |
| Zdroj: | Virology. 308:114-127 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/s0042-6822(03)00198-3 |
| Popis: | The entire genome of SfV, a temperate serotype-converting bacteriophage of Shigella flexneri, has recently been sequenced (Allison, G.E., Angeles, D., Tran-Dinh, N., Verma, N.K. 2002, J. Bacteriol. 184, 1974–1987). Based on the sequence analysis, we further characterised the SfV virion structure and morphogenesis. Electron microscopy indicated that SfV belongs to the Myoviridae morphology family. Analysis of the proteins encoded by orf1, orf2, and orf3 revealed that they were homologous to small and large terminase subunits, and portal proteins, respectively; the protein encoded by orf5 showed homology to capsid proteins. Western immunoblot of the phage with anti-SfV sera revealed two antigenic proteins, and the N-terminal amino acid sequence of the 32-kDa protein corresponded to amino acids 116 to 125 of the ORF5 protein, suggesting that the capsid may be processed. Functional analysis of orf4 showed that it encodes the phage capsid protease. The proteins encoded by orfs1, 2, 3, 4, and 5 are homologous to similar proteins in the Siphoviridae phage family of both gram-positive and gram-negative origin. The capsid and morphogenesis genes are upstream and adjacent to the genes encoding Myoviridae (Mu-like) tail proteins. The organisation of the structural genes of SfV is therefore unique as the head and tail genes originate from different morphology groups. |
| Databáze: | OpenAIRE |
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