A DNA gyrase-binding site at the center of the bacteriophage Mu genome is required for efficient replicative transposition
| Autor: | Martin L. Pato, M M Howe, N P Higgins |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA Replication
Replicative transposition Molecular Sequence Data Restriction Mapping Biology Virus Replication DNA gyrase Bacteriophage mu chemistry.chemical_compound Binding site Prophage Binding Sites Multidisciplinary Base Sequence DNA Superhelical DNA replication Molecular biology DNA Topoisomerases Type II chemistry DNA Viral Mutation DNA Transposable Elements Bacteriophage Mu DNA polymerase mu DNA Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 87:8716-8720 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.87.22.8716 |
| Popis: | We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for greater than 1 hr after induction. |
| Databáze: | OpenAIRE |
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