Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
| Autor: | Caroline Conci Campos, Ana Claudia Souza Rodrigues, Yanara Miranda Ferreira, Isadora Nascimento Rezende, Cláudio Marcos Rocha-de-Souza, Marilene Rodrigues Chang, Ana Paula D'Alincourt Carvalho-Assef, Ivson Cassiano de Oliveira Santos, Claudia Elisabeth Volpe Chaves |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Klebsiella pneumoniae medicine.drug_class Polymyxin 030231 tropical medicine lcsh:QR1-502 Drug resistance polymyxins Polymerase Chain Reaction lcsh:Microbiology law.invention Microbiology 03 medical and health sciences 0302 clinical medicine multidrug resistance law Drug Resistance Multiple Bacterial polycyclic compounds medicine Humans colistin Polymerase chain reaction biology Biodiversity biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Klebsiella Infections Multiple drug resistance Carbapenems Mutation Colistin Multilocus sequence typing Original Article Polymyxin B medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 114, Article number: e180555, Published: 20 MAY 2019 Memórias do Instituto Oswaldo Cruz., Vol 114, Iss 0 (2019) |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/0074-02760180555 |
| Popis: | Background Polymyxins are currently used as a "last-line" treatment for multidrug-resistant Gram-negative infections. Objectives To identify the major mechanisms of resistance to polymyxin and compare the genetic similarity between multi-drug resistant Klebsiella pneumoniae strains recovered from inpatients of public hospitals in the Mid-West of Brazil. Methods 97 carbapenems non-susceptible K. pneumoniae were studied. β-lactamases (bla OXA-48, bla KPC, bla NDM, bla CTX-M, bla SHV, bla TEM, bla IMP, bla VIM) and mcr-1 to mcr-5 genes were investigated by polymerase chain reaction (PCR). Mutations in chromosomal genes (pmrA, pmrB, phoP, phoQ, and mgrB) were screened by PCR and DNA sequencing. Clonal relatedness was established by using pulsed-field gel electrophoresis and multilocus sequence typing. Findings K. pneumoniae isolates harbored bla KPC (93.3%), bla SHV (86.6%), bla TEM (80.0%), bla CTX-M (60%) genes. Of 15 K. pneumoniae resistant to polymyxin B the authors identified deleterious mutations in pmrB gene, mainly in T157P. None K. pneumoniae presented mcr gene variants. Genetic polymorphism analyses revealed 12 different pulsotypes. Main conclusions Deleterious mutations in pmrB gene is the main chromosomal target for induction of polymyxin resistance in carbapenem-resistant K. pneumoniae in public hospitals in the Mid-West of Brazil. |
| Databáze: | OpenAIRE |
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