Lactate Dehydrogenase Levels in the Saliva of Cigarette and E-Cigarette Smokers (Vapers): A Comparative Analysis
| Autor: | Anitha Krishnan Pandarathodiyil, Saman Warnakulasuriya, Jennifer Geraldine Doss, Wan Maria Nabillah Ghani, Anand Ramanathan, Fazliny Binti Abd Rahman, Ranjana Garg |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Saliva Electronic Nicotine Delivery Systems Sensitivity and Specificity chemistry.chemical_compound Young Adult Internal medicine Lactate dehydrogenase medicine Humans Oral mucosa Whole saliva Aged Smokers biology L-Lactate Dehydrogenase business.industry Significant difference General Medicine Non-Smokers Tobacco Products Middle Aged Enzyme assay medicine.anatomical_structure Endocrinology chemistry ROC Curve Area Under Curve biology.protein Oral health care Female Ldh activity business |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 22(10) |
| ISSN: | 2476-762X |
| Popis: | Background We examined the lactate dehydrogenase (LDH) enzyme levels in the saliva of vapers (e-cigarette users) and compared the data with cigarette smokers and a control group of non-smokers and non-vapers. Methods Subjects were recruited among those responding to a social media announcement or patients attending the SEGi Oral Health Care Centre between May and December 2019, and among some staff at the centre. Five ml of unstimulated whole saliva was collected and salivary LDH enzyme activity levels were measured with a LDH colorimetric assay kit. Salivary LDH activity level was determined for each group and compared statistically. Results Eighty-eight subjects were categorized into three groups (control n=30, smokers n=29, and vapers n=29). The mean ± standard deviation (SD) values for salivary LDH activity levels for vapers, smokers, and control groups were 35.15 ± 24.34 mU/ml, 30.82 ± 20.73 mU/ml, and 21.45 ± 15.30 mU/ml, respectively. The salivary LDH activity levels of smoker and vaper groups were significantly higher than in the control group (p = 0.031; 0.017). There was no significant difference of salivary LDH activity level in vapers when compared with smokers (p= 0.234). Conclusion Our findings showed higher LDH levels in the saliva of vapers when compared with controls, confirming cytotoxic and harmful effects of e-cigarettes on the oral mucosa. |
| Databáze: | OpenAIRE |
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