Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus
| Autor: | Hisanori Bando, Shin-ichiro Asano, Ryousuke Fujita, Yasuyuki Nishijima, Takumi Abe, Ken Sahara, Norifumi Miyake |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Gene Expression Regulation
Viral Cancer Research animal structures Transcription Genetic viruses Molecular Sequence Data Sf9 Spodoptera Recombinant virus Primer extension chemistry.chemical_compound Caulimovirus Transcription (biology) Virology RNA polymerase Animals Luciferases Promoter Regions Genetic Cells Cultured Recombination Genetic Base Sequence biology fungi DNA-Directed RNA Polymerases biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Nucleopolyhedroviruses Autographa californica Infectious Diseases chemistry Cauliflower mosaic virus |
| Zdroj: | Virus Research. 112:38-41 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2005.03.019 |
| Popis: | We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase. |
| Databáze: | OpenAIRE |
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