The Effects of Lactobacillus rhamnosus on the Prevention of Asthma in a Murine Model
| Autor: | Seong Ok Jang, Soo-Jong Hong, Jinho Yu, Won Ah Choi, Ji Won Kwon, Hyun Don Jung, Byoung-Ju Kim, Young Hwa Song, Mi Jin Kang |
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| Rok vydání: | 2010 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lactobacillus casei Immunology primary prevention Immunoglobulin E Blood serum Lactobacillus rhamnosus medicine Immunology and Allergy Sensitization Asthma medicine.diagnostic_test biology business.industry respiratory system biology.organism_classification medicine.disease respiratory tract diseases medicine.anatomical_structure Bronchoalveolar lavage probiotics biology.protein Methacholine Original Article business animal disease model medicine.drug |
| Zdroj: | Allergy, Asthma & Immunology Research |
| ISSN: | 2092-7363 |
| Popis: | PURPOSE: Lactobacilli are probiotic bacteria that are effective in the management of allergic diseases or gastroenteritis. It is hypothesized that such probiotics have immunoregulatory properties and promote mucosal tolerance. Our goal was to investigate whether Lactobacillus casei rhamnosus Lcr35 could inhibit airway inflammation in an ovalbumin (OVA)-induced murine model of asthma. METHODS: BALB/c mice aged 6 weeks were used in the present study. Lactobacillus casei rhamnosus Lcr35 was administered daily, starting 1 week prior to the first OVA sensitization (group 1) and 2 days before the first 1% OVA airway challenge (group 2). Mice that received only saline at both sensitization and airway challenge time points were used as negative controls (group 3), and those that had OVA-induced asthma were used as positive controls (group 4). Airway responsiveness to methacholine was assessed, and bronchoalveolar lavage (BAL) was performed. At the endpoint of the study, total IgE as well as OVA-specific IgE, IgG(1) and IgG(2a) in serum was measured by enzyme-linked immunosorbent assay. Lung pathology was also evaluated. RESULTS: Airway hyperresponsiveness, total cell counts and the proportion of eosinophils in BAL fluid were significantly decreased in group 1 compared with group 4 (P |
| Databáze: | OpenAIRE |
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