Amphiregulin is an implantation-specific and progesterone-regulated gene in the mouse uterus

Autor: Sanjoy K. Das, I Chakraborty, G.D. Plowman, Xiaohong Wang, Sudhansu K. Dey, Bibhash C. Paria
Rok vydání: 1995
Předmět:
Male
medicine.medical_treatment
Uterus
Dexamethasone
Mice
Endocrinology
Pregnancy
Epidermal growth factor
Cloning
Molecular
Phosphorylation
Pseudopregnancy
Gonadal Steroid Hormones
Growth Substances
Progesterone
Nucleic Acid Hybridization
General Medicine
ErbB Receptors
Mifepristone
medicine.anatomical_structure
Ovariectomized rat
Intercellular Signaling Peptides and Proteins
Female
EGF Family of Proteins
medicine.medical_specialty
medicine.drug_class
Ovariectomy
Molecular Sequence Data
Gestational Age
Biology
Amphiregulin
Andrology
Estrus
Internal medicine
medicine
Animals
Amino Acid Sequence
Embryo Implantation
Blastocyst
Molecular Biology
Glycoproteins
Messenger RNA
Base Sequence
Growth factor
Epithelial Cells
Gene Expression Regulation
Genes
Estrogen
Protein Processing
Post-Translational
Zdroj: Molecular Endocrinology. 9:691-705
ISSN: 1944-9917
0888-8809
Popis: A synchrony between the activated state of the blastocyst and differentiation of the uterus to the receptive state is essential to the process of implantation. This process is directed by progesterone (P4) and estrogen. The mechanism by which P4 differentiates the uterus, enabling estrogen to initiate implantation, is unknown but likely to involve localized induction of growth and differentiation factors. We have cloned the murine amphiregulin (AR) gene, a newly discovered member of the epidermal growth factor family, and demonstrate that its expression is implantation-specific and P4-regulated in the mouse uterus. A transient surge in AR mRNA levels occurred throughout the uterine epithelium on day 4 of pregnancy. With the onset of blastocyst attachment late on day 4, AR mRNA accumulated in the luminal epithelium exclusively at the sites of blastocysts. Thus, AR expression correlated first with rising P4 levels and then with the attachment reaction. The rapid induction of AR mRNA in the ovariectomized uterus only by P4 and abrogation of this induction by RU-486 (a P4 receptor antagonist) suggest that this uterine gene is regulated by P4. AR appeared to exhibit preferential phosphorylation of epidermal growth factor receptor in the uterus over that in the blastocyst. This is a first report of a P4-regulated uterine epithelial cell growth factor that is associated with epithelial cell differentiation during implantation. The association of AR in implantation is further documented by its down-regulation in the day 4 pregnant uterus in which uterine receptivity and implantation were disrupted by estrogen or RU-486 treatment on day 3. These results further indicate that the expression of the AR gene could serve as a molecular marker for the receptive state of the uterus for implantation.
Databáze: OpenAIRE
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