Bone Scaffolds Based on Degradable Vaterite/PEG‐Composite Microgels
| Autor: | Alena Kuzmina, Romina Schröder, Elena Stengelin, Sebastian Seiffert, Wolfgang Tremel, Laura Besch, Martha F. Koziol, Ronald E. Unger, Guillermo Beltramo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biocompatibility
Dispersity Biomedical Engineering Pharmaceutical Science 02 engineering and technology 010402 general chemistry Bone tissue 01 natural sciences Bone and Bones Calcium Carbonate Biomaterials chemistry.chemical_compound Osteogenesis Vaterite PEG ratio medicine ddc:610 chemistry.chemical_classification Microgels Osteoblast Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure chemistry Chemical engineering 0210 nano-technology Gels Ethylene glycol |
| Zdroj: | Advanced healthcare materials 9(11), 1901820 (2020). doi:10.1002/adhm.201901820 |
| DOI: | 10.1002/adhm.201901820 |
| Popis: | Vaterite, a metastable modification of calcium carbonate, embedded in a flexible microgel packaging with adjustable mechanical properties, functionality, and biocompatibility, provides a powerful scaffolding for bone tissue regeneration, as it is easily convertible to bone-like hydroxyapatite (HA). In this study, the synthesis and physical analysis of a packaging material to encapsulate vaterite particles and osteoblast cells into monodisperse, sub-millimeter-sized microgels, is described whereby a systematic approach is used to tailor the microgel properties. The size and shape of the microgels is controlled via droplet-based microfluidics. Key requirements for the polymer system, such as absence of cytotoxicity as well as biocompatibility and biodegradability, are accomplished with functionalized poly(ethylene glycol) (PEG), which reacts in a cytocompatible thiol-ene Michael addition. On a mesoscopic level, the microgel stiffness and gelation times are adjusted to obtain high cellular viabilities. The co-encapsulation of living cells provides i) an in vitro platform for the study of cellular metabolic processes which can be applied to bone formation and ii) an in vitro foundation for novel tissue-regenerative therapies. Finally, the degradability of the microgels at physiological conditions caused by hydrolysis-sensitive ester groups in the polymer network is examined. |
| Databáze: | OpenAIRE |
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