Characterization of secretomes provides evidence for adipose-derived mesenchymal stromal cells subtypes
| Autor: | Dmitry S. Ischenko, Olga Grigorieva, Ksenia Rubina, Veronika Yu. Sysoeva, Olga Pobeguts, Vadim M. Govorun, Ivan Butenko, George V. Sharonov, Natalia Kalinina, Anastasia Efimenko, Dmitry G. Alekseev, Vassiliy Lazarev, Luidmila Ageeva, Daria Kharlampieva, Marina Loguinova |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Secretome profiling Cellular differentiation Medicine (miscellaneous) Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Cell therapy Immunophenotyping Extracellular matrix Neurotrophic factors Ribonuclease 4 Regeneration Humans Secretion Cells Cultured Hypoxic response Research Secretory Vesicles Mesenchymal stem cell Adipose-derived mesenchymal stromal cells Proteins Cell Differentiation Mesenchymal Stem Cells Cell Biology Middle Aged Cell Hypoxia Cell biology Secretory protein Adipose Tissue Culture Media Conditioned Molecular Medicine Female Stem cell |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| Popis: | Introduction This study was aimed at deciphering the secretome of adipose-derived mesenchymal stromal cells (ADSCs) cultured in standard and hypoxic conditions to reveal proteins, which may be responsible for regenerative action of these cells. Methods Human ADSCs were isolated from 10 healthy donors and cultured for 3–4 passages. Cells were serum deprived and cell purity was assessed using multiple cell surface markers. Conditioned media was collected and analyzed using LC-MS with a focus on characterizing secreted proteins. Results Purity of the ADSC assessed as CD90+/CD73+/CD105+/CD45-/CD31- cells was greater than 99 % and viability was greater than 97 %. More than 600 secreted proteins were detected in conditioned media of ADSCs. Of these 100 proteins were common to all cultures and included key molecules involved in tissue regeneration such as collagens and collagen maturation enzymes, matrix metalloproteases, matricellular proteins, macrophage-colony stimulating factor and pigment epithelium derived factor. Common set of proteins also included molecules, which contribute to regenerative processes but were not previously associated with ADSCs. These included olfactomedin-like 3, follistatin-like 1 and prosaposin. In addition, ADSCs from the different subjects secreted proteins, which were variable between different cultures. These included proteins with neurotrophic activities, which were not previously associated with ADSCs, such as mesencephalic astrocyte-derived neurotrophic factor, meteorin and neuron derived neurotrophic factor. Hypoxia resulted in secretion of 6 proteins, the most prominent included EGF-like repeats and discoidin I-like domains 3, adrenomedullin and ribonuclease 4 of RNase A family. It also caused the disappearance of 8 proteins, including regulator of osteogenic differentiation cartilage-associated protein. Conclusions Human ADSCs with CD90+/CD73+/CD105+/CD45-/CD31-/PDGFRβ+/NG2+/CD146+(−) immunophenotype secrete a large array of proteins, the most represented group is comprised of extracellular matrix components. Number of secreted proteins is largely unaffected by prolonged hypoxia. Variability in the secretion of several proteins from cultured ADSCs of individual subjects suggests that these cells exist as a heterogeneous population containing functionally distinct subtypes, which differ in numbers between donors. |
| Databáze: | OpenAIRE |
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