The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice

Autor: Yoko Kita, Hiroko Takai, Yoshinobu Okuno, Daphne E. deMello, Yumiko Muraki, Takao Tanaka, Shigeto Yoshida, Masaji Okada, Yuji Takemoto, Yayoi Sakaguchi, Yoshiyuki Yoshinaka, Kyoko Yamada, Chika Okada, Masato Tashiro, Tetsuo Kase, Naoki Yamamoto, Shigeru Morikawa, Joseph S. M. Peiris, Yukari Fukunaga, Tatsuji Nomura, Pei-Jer Chen, Sachiko Kuwayama, Noriko Kanamaru, Isao Ishida, Satomi Hashimoto, Makoto Matsumoto, Izumi Furukawa, Mitsunori Sakatani
Rok vydání: 2005
Předmět:
T cell
Transplantation
Heterologous
chemical and pharmacologic phenomena
Mice
SCID
In Vitro Techniques
Antibodies
Viral
Lymphocyte Activation
medicine.disease_cause
Article
Virus
DNA vaccination
Mice
Viral Proteins
Immune system
Antigen
Mice
Inbred NOD
Neutralization Tests
Vaccines
DNA
medicine
Animals
Humans
skin and connective tissue diseases
Antigens
Viral
Coronavirus
Mice
Knockout
Mice
Inbred BALB C
General Veterinary
General Immunology and Microbiology
biology
fungi
Public Health
Environmental and Occupational Health
Viral Vaccines
Virology
Mice
Inbred C57BL
body regions
CTL
SCID-PBL/hu
Infectious Diseases
medicine.anatomical_structure
Severe acute respiratory syndrome-related coronavirus
Lymphocyte Transfusion
biology.protein
Human CTL
Molecular Medicine
Female
SARS DNA vaccine
Antibody
T-Lymphocytes
Cytotoxic
Zdroj: Vaccine
ISSN: 0264-410X
Popis: We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.
Databáze: OpenAIRE
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