Podosomes are dispensable for osteoclast differentiation and migration
| Autor: | Heiani Touaitahuata, Géraldine Pawlak, Corinne Albiges-Rizo, Anne Blangy, Emmanuelle Planus |
|---|---|
| Přispěvatelé: | Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
musculoskeletal diseases
Integrins Histology Podosome Cellular differentiation [SDV]Life Sciences [q-bio] Integrin Acrylic Resins Osteoclasts [SDV.BC]Life Sciences [q-bio]/Cellular Biology Pathology and Forensic Medicine Cell-Matrix Junctions Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Osteoclast Cell Movement medicine Cell Adhesion Animals Dimethylpolysiloxanes Cell adhesion Cells Cultured ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Chemistry Cell Differentiation Cell Biology General Medicine Vinculin Actins Cell biology Culture Media Extracellular Matrix Mice Inbred C57BL Agar medicine.anatomical_structure src-Family Kinases 030220 oncology & carcinogenesis Podosome assembly biology.protein |
| Zdroj: | European Journal of Cell Biology European Journal of Cell Biology, Elsevier, 2013, 92 (4-5), pp.139-149. ⟨10.1016/j.ejcb.2013.03.001⟩ |
| ISSN: | 0171-9335 |
| DOI: | 10.1016/j.ejcb.2013.03.001⟩ |
| Popis: | Podosomes are adhesion structures characteristic of the myeloid cell lineage, encompassing osteoclasts, dendritic cells and macrophages. Podosomes are actin-based structures that are dynamic and capable of self-organization. In particular in the osteoclast, podosomes densely pack into a thick ring called the sealing zone. This adhesion structure is typical of osteoclasts and necessary for the resorption of the bone matrix. We thought to explore in more details the role of podosomes during osteoclast differentiation and migration. To this end, we made from soft to stiff substrates that had not been functionalized with extracellular matrix proteins. Such substrates did not support podosome formation in osteoclasts. With such devices, we could show that integrin activation was sufficient to drive podosome assembly, in a substrate stiffness independent fashion. We additionally report here that osteoclast differentiation is a podosome-independent process. Finally, we show that osteoclasts devoid of podosomes can migrate efficiently. Our study further illustrates the great capacity of myeloid cells to adapt to the different environments they encounter during their life cycle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect