Stability of EPSPS gene copy number in Hordeum glaucum Steud (barley grass) in the presence and absence of glyphosate selection
Autor: | Jenna Malone, Christopher Preston, Gurjeet Gill, Patricia Adu-Yeboah |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
clone (Java method) Gene Dosage Glycine Biology 01 natural sciences Phosphates chemistry.chemical_compound South Australia Gene duplication Copy-number variation Gene Selection (genetic algorithm) Herbicides musculoskeletal neural and ocular physiology Hordeum General Medicine biology.organism_classification 010602 entomology Horticulture nervous system chemistry Insect Science Glyphosate 3-Phosphoshikimate 1-Carboxyvinyltransferase Weed Agronomy and Crop Science Herbicide Resistance 010606 plant biology & botany |
Zdroj: | Pest Management Science. 77:3080-3087 |
ISSN: | 1526-4998 1526-498X |
DOI: | 10.1002/ps.6367 |
Popis: | BACKGROUND Gene amplification has been shown to provide resistance to glyphosate in several weed species, including Hordeum glaucum populations in South Australia. The stability of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene copies in resistant populations in the presence or absence of glyphosate selection has not been determined. RESULTS Applying glyphosate to a cloned plant resulted in an increase in resistance and EPSPS copy number in the progeny of that plant compared to the untreated clone. The LD50 (herbicide concentration required for 50% mortality) increased by 75% to 79% in the progeny of the treated clones compared to the untreated in both populations (YP-17 and YP-16). EPSPS copy number estimates were higher in treated individuals compared to untreated individuals with an average of seven copies compared to six in YP-16 and 11 compared to six in YP-17. There was a positive correlation (R2 = 0.78) between EPSPS copy number and LD50 of all populations. CONCLUSION EPSPS gene copy number and resistance to glyphosate increased in H. glaucum populations under glyphosate selection, suggesting the number of EPSPS gene copies present is dependent on glyphosate selection. © 2021 Society of Chemical Industry. |
Databáze: | OpenAIRE |
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