Analyses of carcinogenic aromatic amines released from harmful azo colorants by Streptomyces SP. SS07
| Autor: | Ganga Radhakrishnan, Sayeed Sadulla, Raghavan Chandrasekar, R. Ganeshjeevan, Arumugam Gnanamani, Malayappan Bhaskar |
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| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Organic Chemistry Xylidine Aromatic amine General Medicine biology.organism_classification Biochemistry Streptomyces High-performance liquid chromatography Benzidine Analytical Chemistry chemistry.chemical_compound chemistry Carcinogens Organic chemistry Spectrophotometry Ultraviolet Amine gas treating Gas chromatography Amines Azo Compounds Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1018:117-123 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2003.08.024 |
| Popis: | Extracellular fluid protein (ECFP) of Streptomyces species SS07 has been used to reduce water soluble azo dyes and the carcinogenic amines released have been compared with that from chemical reduction. The effect of temperature, pH and contact time on the recovery of amines using ECFP was studied. The ECFP releases carcinogenic amines at a pH of 9.2 and a temperature of 37 °C for a contact period of 24 h. The reduction products were analyzed with HPLC and their structures confirmed by LC–MS and GC–MS. It was observed that both the ECFP and chemical reduction methods released similar type of amine products. In the case of dye samples, compared to chemical reduction, 5–20% increase in the release of carcinogenic amines by ECFP was observed. The percentage of amine products released by chemical reduction was higher for leather garment samples compared to ECFP treatment. |
| Databáze: | OpenAIRE |
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