In vivo characterization of minipig skin as a model for dermatological research using multiphoton microscopy

Autor:	Darold R. Spillman, Eric J. Chaney, Joanne Li, Marina Marjanovic, Phaedra B. Hutchison, Stephen A. Boppart, Steve Frey, Zane Arp, Mantas Žurauskas, Jennifer M. Criley, Aneesh Alex
Rok vydání:	2020
Předmět:	0301 basic medicine chemistry.chemical_classification Fluorescence-lifetime imaging microscopy integumentary system biology Human skin Dermatology Optical Biopsy Biochemistry Article Characterization (materials science) 030207 dermatology & venereal diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine chemistry In vivo Keratin biology.protein Molecular Biology Elastin Preclinical imaging Biomedical engineering
Zdroj:	Exp Dermatol
ISSN:	1600-0625 0906-6705
DOI:	10.1111/exd.14152
Popis:	Minipig skin is one of the most widely used non-rodent animal skin models for dermatological research. A thorough characterization of minipig skin is essential for gaining deeper understanding of its structural and functional similarities with human skin. In this study, three-dimensional (3-D) in vivo images of minipig skin was obtained non-invasively using a multimodal optical imaging system capable of acquiring two-photon excited fluorescence (TPEF) and fluorescence lifetime imaging microscopy (FLIM) images simultaneously. The images of the structural features of different layers of the minipig skin were qualitatively and quantitatively compared with those of human skin. Label-free imaging of skin was possible due to the endogenous fluorescence and optical properties of various components in the skin such as keratin, nicotinamide adenine dinucleotide phosphate (NAD(P)H), melanin, elastin, and collagen. This study demonstrates the capability of optical biopsy techniques, such as TPEF and FLIM, for in vivo non-invasive characterization of cellular and functional features of minipig skin, and the optical image-based similarities of this commonly utilized model of human skin. These optical imaging techniques have the potential to become promising tools in dermatological research for developing a better understanding of animal skin models, and for aiding in translational pre-clinical to clinical studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bd21adbe7940993512644c5fd2ff8f8 https://doi.org/10.1111/exd.14152 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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