Gene expression profiling of acute graft-vs-host disease after hematopoietic stem cell transplantation
| Autor: | Jan Verner, Zbynek Zdrahal, Marek Mráz, Šárka Pospíšilová, Jana Volejnikova, Michael Doubek, Marta Krejčí, Yvona Brychtová, Alexandra Tomancová, Boris Tichy, Šárka Pavlová, Jiri Mayer, Martin Trbušek, Jitka Kabáthová, Petr Sedlacek |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_treatment Graft vs Host Disease Disease Hematopoietic stem cell transplantation Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine Immune system immune system diseases hemic and lymphatic diseases Genetics Medicine Humans Child Molecular Biology Survival rate 030304 developmental biology 0303 health sciences business.industry Gene Expression Profiling Hematopoietic Stem Cell Transplantation Cell Biology Hematology Cell cycle Middle Aged 3. Good health Gene expression profiling Survival Rate surgical procedures operative Cytokine 030220 oncology & carcinogenesis Child Preschool Immunology Acute Disease Female business |
| Zdroj: | Experimental hematology. 40(11) |
| ISSN: | 1873-2399 |
| Popis: | Acute graft-vs-host disease (aGVHD) is a frequent, life-threatening complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Despite that, there are no reliable molecular markers reflecting the onset or clinical course of aGVHD. We performed a pilot study on gene expression profiling in peripheral blood mononuclear cells taken from 15 patients with hematological malignancies who underwent allo-HSCT and developed aGVHD. Based on survival rates after aGVHD, patients were divided into two groups—favorable (all patients alive; median follow-up 40 months) vs unfavorable group (all patients died; median survival 2 months). Two-hundred and eighty genes differentially expressed between these two groups were identified; among them, genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle were over-represented; interleukin-8 , G0S2 , ANXA3 , and NR4A2 were upregulated in the unfavorable group, CDKN1C was downregulated in the same group. Interestingly, the same genes were also described as overexpressed in connection with autoimmune diseases. This indicates an involvement of similar immune regulatory pathways also in aGVHD. Our data support use of gene expression profiling at aGVHD onset for a prediction of its outcomes. |
| Databáze: | OpenAIRE |
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