Lipopolysaccharide increases endogenous morphine levels in rat brain
| Autor: | Yannick Goumon, Jean-Claude Beauvillain, Sebastien G. Bouret, George B. Stefano, Frederico Casares, Wei Zhu |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Lipopolysaccharide Dependent manner medicine.medical_treatment Intraperitoneal injection Endogeny Post injection High-performance liquid chromatography Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Internal medicine medicine Electrochemistry Animals Chromatography High Pressure Liquid Brain Chemistry Morphine General Neuroscience Brain Rat brain Rats Endocrinology chemistry Female Injections Intraperitoneal medicine.drug |
| Zdroj: | Neuroscience letters. 293(2) |
| ISSN: | 0304-3940 |
| Popis: | The present study was designed to determine whether whole body injection of lipopolysaccharide increases endogenous rat brain morphine levels. High performance liquid chromatography coupled to electrochemical detection and gas-chromatography mass spectrometry reveal that the resting brain morphine level is 7.0+/-3.2ng/g wet weight. In a time dependent manner, intraperitoneal injection of lipopolysaccharide (10 microg), leads to an increase of brain morphine level with a peak reaching after 36h post injection. After 72h following the lipopolysaccharide-injection, morphine levels increased to 66.0+/-5.4ng/g brain wet weight (P0.001). Furthermore, 96h of fasting also increased the brain morphine level to 44.0+/-3. 6ng/g of brain wet weight. These results suggest that rat brain morphinergic processes respond to organismic challenges after a significant latent period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect