Serum Autofluorescence and Biochemical Markers in Athlete’s Response to Strength Effort in Normobaric Hypoxia: A Preliminary Study
| Autor: | Anna Michnik, Izabela Schisler, Zofia Drzazga, Stanisław Poprzęcki, Miłosz Czuba |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Article Subject Strength training Normobaric Hypoxia lcsh:Medicine Creatine General Biochemistry Genetics and Molecular Biology Biochemical Markers Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endurance training Altitude training Internal medicine medicine Humans Lactic Acid Hypoxia Fluorescence spectroscopy Creatine Kinase Exercise General Immunology and Microbiology Chemistry Altitude lcsh:R Assay 030229 sport sciences General Medicine Effects of high altitude on humans Hypoxia (medical) Oxygen Autofluorescence Spectrometry Fluorescence 030104 developmental biology Endocrinology Athletes medicine.symptom Biomarkers Research Article |
| Zdroj: | BioMed Research International, Vol 2019 (2019) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | The human organism has the ability to adapt to hypoxia conditions. Training in hypoxia is used in sport to improve the efficiency of athletes; however, type of training affects the direction and scope of this process. Therefore, in this study, the usefulness of serum fluorescence spectroscopy to study the assessment of athlete’s response to strength effort in hypoxia is considered in comparison with biochemical assay. Six resistance-trained male subjects took part in a research experiment. They performed barbell squats in simulated normobaric hypoxic conditions with deficiency of oxygen 11.3%, 13% 14.3% compared to 21% in normoxic conditions. Fluorescence intensity of tyrosine revealed high sensitivity on strength effort whereas tryptophan was more dependent on high altitude. Changes in emission in the visible region are associated with altering cell metabolism dependent on high altitude as well as strength training and endurance training. Significant changes in serum fluorescence intensity with relatively weak modifications in biochemical assay at 3000 m above sea level (ASL) were observed. Training at 5000 m ASL caused changes in fluorescence parameters towards the normobaric specific values, and pronounced decreases of lactate level and kinase creatine activity were observed. Such modifications of fluorescence and biochemical assay indicate increased adaptation of the organism to effort in oxygen-deficient conditions at 5000 m ASL, unlike 3000 m ASL. Fluorescence spectroscopy study of serum accompanied by biochemical assay can contribute to the understanding of metabolic regulation and the physiological response to hypoxia. The results of serum autofluorescence during various concepts of altitude training may be a useful method to analyze individual response to acute and chronic hypoxia. An endogenous tryptophan could be exploited as intrinsic biomarker in autofluorescence studies. However, these issues require further research. |
| Databáze: | OpenAIRE |
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