Maternal overweight induced by reduced litter size impairs the behavioral neurodevelopment of offspring
| Autor: | Fernando Vitor-Vieira, Fabiana C. Vilela, Viviana Carolina Trujillo Rojas, Tatiane Helena Batista, Alexandre Giusti-Paiva, Jádina S. Vieira, Cíntia O. de Novais, Bárbara G. Ferri, Ana Cláudia Alves Freire Ribeiro |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Litter (animal) Litter Size Offspring Physiology Hippocampus Biology Overweight 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Open field 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Animals Nutritional Physiological Phenomena Rats Wistar General Pharmacology Toxicology and Pharmaceutics Maternal Behavior Prefrontal cortex Homing (biology) Body Weight Brain General Medicine Rats 030104 developmental biology Animals Newborn Neurodevelopmental Disorders Prenatal Exposure Delayed Effects Synaptophysin biology.protein Female medicine.symptom |
| Zdroj: | Life Sciences. 277:119611 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2021.119611 |
| Popis: | Aims We assessed the influence of maternal overweight on the behavioral neurodevelopment of male and female offspring in prepubertal age by reducing the litter size. Main methods To reduce litter size in Wistar rats, the offspring of generation 0 (G0) were culled for 12 pups (6 males and 6 females: normal litter, NL-G1) or 4 pups (2 males and 2 females: small litter, SL-G1). In G1 dams, overweight was characterized, maternal behavior and locomotor activity were assessed. At G2, we quantified the ultrasonic vocalizations in post-natal day 5 (PND5); we evaluated olfactory discrimination in the homing behavior test on PND13; and in PND28–32 (prepubertal age), we performed the following tests: social play behavior, hole board, object recognition, and open field. At the end of the experiments, hippocampus and prefrontal cortex were dissected to quantify the synaptophysin by western blotting. Key findings Our data demonstrated that a reduction in litter size was able to induce maternal overweight without altering the parameters related to overweight in the offspring. The SL-G2 offspring showed deficits in early social communication, olfactory discrimination, social play behavior, and the exploration of objects, in addition to increasing repetitive and stereotyped movements. There were also changes in the synaptophysin levels in the hippocampus and prefrontal cortex of the offspring from reduced litter dams. In conclusion, maternal overweight caused by litter reduction impairs behavioral neurodevelopment, inducing autism-like symptoms in the offspring. Significance This study alerts the public about the negative consequences of maternal overweight in the descendants. |
| Databáze: | OpenAIRE |
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