CCAAT/enhancer-binding protein (C/EBP) immunoreactivity during rat liver carcinogenesis
| Autor: | B. Lindeman, Ellen Skarpen, Marit Låg, Thoresen Gh, Henrik S. Huitfeldt, Thoralf Christoffersen |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Histology Cellular differentiation Blotting Western Down-Regulation Cell Separation Biology medicine.disease_cause chemistry.chemical_compound Liver Neoplasms Experimental medicine Tumor Cells Cultured Animals Rats Wistar Molecular Biology Transcription factor Microscopy Confocal Ccaat-enhancer-binding proteins Nuclear Proteins Cell Biology 2-Acetylaminofluorene Molecular biology Immunohistochemistry Liver regeneration Rats Inbred F344 Liver Regeneration Rats Blot DNA-Binding Proteins Medical Laboratory Technology medicine.anatomical_structure Enhancer Elements Genetic chemistry Fluorescent Antibody Technique Direct Hepatocyte CCAAT-Enhancer-Binding Proteins Carcinogens Carcinogenesis Precancerous Conditions Transcription Factors |
| Zdroj: | Histochemistry and cell biology. 104(4) |
| ISSN: | 0948-6143 |
| Popis: | To elucidate cell differentiation in liver carcinogenesis, we have studied the CCAAT/enhancer-binding protein (C/EBP). C/EBP is a positive-acting transcription factor important for the maintenance of liverspecific functions. It is associated with differentiation and regarded as an anti-proliferative agent. We have studied the expression and localization of C/EBP during sequential rat liver carcinogenesis. Two-color immuno-histochemistry and confocal laser scan microscopy demonstrated C/EBP in hepatocyte nuclei and preneoplastic liver lesions, but not in bile ducts, non-parenchymal cells or oval cells. Both western blotting and immunohistochemistry revealed down-regulation of C/EBP during normal regeneration and when regeneration was inhibited by the carcinogen, 2-acetylaminofluorene. A similar down-regulation was shown by western blotting in hepatocytes grown in culture. Our data suggest that the altered metabolic phenotype of preneoplastic liver lesions was not caused by a change in the expression of C/EBP. Furthermore, the data favor a hepatocyte derivation of preneoplastic liver lesions. |
| Databáze: | OpenAIRE |
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