Temporal regulation of HTLV-2 expression in infected cell lines and patients: evidence for distinct expression kinetics with nuclear accumulation of APH-2 mRNA
| Autor: | Cecilia Bender, Ilaria Cavallari, Francesca Rende, Alessia Cotena, Paola Righi, Vincenzo Ciminale, Claudio Casoli, Umberto Bertazzoni, Paola Ronzi |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Gene Expression Regulation Viral Male RNA Splicing viruses Short Report Peripheral blood mononuclear cell In vivo immune system diseases Virology medicine Humans Cells Cultured Messenger RNA Human T-lymphotropic virus 1 biology Human T-lymphotropic virus 2 virus diseases Middle Aged HTLV biology.organism_classification medicine.disease Molecular biology In vitro Leukemia Infectious Diseases biology.protein Leukocytes Mononuclear RNA Viral Female Antibody lcsh:RC581-607 |
| Zdroj: | Retrovirology Retrovirology, Vol 9, Iss 1, p 74 (2012) |
| Popis: | Background Human T-cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2) are delta retroviruses with similar genetic organization. Although both viruses immortalize T-cells in vitro, they exhibit distinct pathogenic potential in vivo. To search for possible differences in its expression strategy with respect to HTLV-1, we investigated the pattern of HTLV-2 expression in infected cell lines and peripheral blood mononuclear cells (PBMCs) from infected patients using splice site-specific quantitative RT-PCR. Findings A novel alternative splice acceptor site for exon 2 was identified; its usage in env transcripts was found to be subtype-specific. Time-course analysis revealed a two-phase expression kinetics in an infected cell line and in PBMCs of two of the three patients examined; this pattern was reminiscent of HTLV-1. In addition, the minus-strand APH2 transcript was mainly detected in the nucleus, a feature that was similar to its HTLV-1 orthologue HBZ. In contrast to HTLV-1, expression of the mRNA encoding the main regulatory proteins Tax and Rex and that of the mRNAs encoding the p28 and truncated Rex inhibitors is skewed towards p28/truncated Rex inhibitors in HTLV-2. Conclusion Our data suggest a general converging pattern of expression of HTLV-2 and HTLV-1 and highlight peculiar differences in the expression of regulatory proteins that might influence the pathobiology of these viruses. |
| Databáze: | OpenAIRE |
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