Detection of DNA damage in stressed trout nucleated erythrocytes using the comet assay: protection by nitroxide radicals
| Autor: | Lucedio Greci, Donatella Fedeli, Giancarlo Falcioni, Anna Maria Santroni, Milena Villarini, Elisabetta Damiani, Massimo Moretti |
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| Rok vydání: | 1998 |
| Předmět: |
Erythrocytes
Free Radicals DNA damage In Vitro Techniques medicine.disease_cause Biochemistry Hemolysis Antioxidants chemistry.chemical_compound Physiology (medical) medicine Animals Gel electrophoresis chemistry.chemical_classification Electrophoresis Agar Gel biology Glutathione peroxidase biology.organism_classification medicine.disease Comet assay Trout Oxidative Stress chemistry Oncorhynchus mykiss Nitrogen Oxides DNA Oxidative stress DNA Damage |
| Zdroj: | Free radical biologymedicine. 24(7-8) |
| ISSN: | 0891-5849 |
| Popis: | Because previous literature reports have demonstrated that nucleated trout erythrocytes in conditions of oxidative stress are subjected to both membrane damage and a decrease in the enzymatic defense systems (glutathione peroxidase), which in turn lead to hemolysis, the present study was undertaken to determine whether DNA may be affected too, prior to the hemolytic event. Impairment of DNA in stressed trout erythrocytes was assessed using the comet assay--a rapid and sensitive, single-cell gel electrophoresis technique used to detect primary DNA damage in individual cells. In addition, indolinic and quinolinic nitroxide radicals were included in the study to determine their efficacy as antioxidants against free-radical-induced DNA damage. The parameters, tail length, tail intensity, and tail moment, used as an index of DNA damage, have shown that trout erythrocytes exposed to oxidative stress experience DNA damage prior to hemolysis and that the nitroxides significantly prevent this damage. This result provides further information about the potential use of these compounds as antioxidants in biological systems. |
| Databáze: | OpenAIRE |
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