Coupling Bioflocculation of Dehalococcoides mccartyi to High-Rate Reductive Dehalogenation of Chlorinated Ethenes
| Autor: | Emily Bondank, Sofia Esquivel-Elizondo, Rosa Krajmalnik-Brown, Devyn Fajardo-Williams, Anca G. Delgado |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification High rate Bioaugmentation 030106 microbiology Dehalococcoides mccartyi Halogenation Chloroflexi General Chemistry Ethylenes 010501 environmental sciences Electron acceptor equipment and supplies 01 natural sciences Trichloroethylene 03 medical and health sciences Continuous treatment Biodegradation Environmental chemistry RNA Ribosomal 16S Environmental chemistry Bioreactor Environmental Chemistry 0105 earth and related environmental sciences |
| Zdroj: | Environmental Science & Technology. 51:11297-11307 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.7b03097 |
| Popis: | Continuous bioreactors operated at low hydraulic retention times have rarely been explored for reductive dehalogenation of chlorinated ethenes. The inability to consistently develop such bioreactors affects the way growth approaches for Dehalococcoides mccartyi bioaugmentation cultures are envisioned. It also affects interpretation of results from in situ continuous treatment processes. We report bioreactor performance and dehalogenation kinetics of a D. mccartyi-containing consortium in an upflow bioreactor. When fed synthetic groundwater at 11–3.6 h HRT, the upflow bioreactor removed >99.7% of the influent trichloroethene (1.5–2.8 mM) and produced ethene as the main product. A trichloroethene removal rate of 98.51 ± 0.05 me– equiv L–1 d–1 was achieved at 3.6 h HRT. D. mccartyi cell densities were 1013 and 1012 16S rRNA gene copies L–1 in the bioflocs and planktonic culture, respectively. When challenged with a feed of natural groundwater containing various competing electron acceptors and 0.3–0.4 mM tri... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|