IL-1α secreted by colon cancer cells enhances angiogenesis: The relationship between IL-1α release and tumor cells' potential for liver metastasis
| Autor: | Hitoshi Funahashi, Hirozumi Sawai, Hiromitsu Takeyama, Donghui Xu, Yoichi Matsuo, Hiroki Takahashi, Nobuo Ochi, Jiachi Ma, Akira Yasuda |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Colorectal cancer Angiogenesis Enzyme-Linked Immunosorbent Assay Metastasis Neovascularization Cell Line Tumor Interleukin-1alpha Humans Medicine Neoplasm Invasiveness RNA Messenger Cell Proliferation Neovascularization Pathologic Reverse Transcriptase Polymerase Chain Reaction business.industry Cell growth Liver Neoplasms General Medicine medicine.disease Oncology Cell culture Colonic Neoplasms Cancer cell Cancer research Surgery Caco-2 Cells medicine.symptom business |
| Zdroj: | Journal of Surgical Oncology. 99:361-367 |
| ISSN: | 1096-9098 0022-4790 |
| Popis: | Background and Objectives Interleukin (IL)-1α plays an important role in colon cancer progression and angiogenesis. We here asked whether IL-1α derived from cancer cells modulates vascular endothelial cell growth, migration and tubule formation. Methods The existence of IL-1α mRNA and protein in colon cancer cell lines (WiDr, HT-29, Caco-2, COLO 320) were investigated with RT-PCR and ELISA. Proliferation and invasion were investigated by MTS assay and Matrigel-double chamber assay. To answer our main question, we performed angiogenesis assay used an in vitro model consisting of co-cultivated tumor cells and stromal cells. Results IL-1α mRNA and protein were detected in highly metastatic colon cancer cells (WiDr and HT-29). Recombinant IL-1α significantly enhanced growth and invasiveness of human umbilical vein endothelial cells (HUVEC) (P |
| Databáze: | OpenAIRE |
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