Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription
| Autor: | Muyu Xu, Ernest Martinez, Elsie Gonzalez-Hurtado |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serum Response Factor Transcription Genetic Regulatory Sequences Nucleic Acid TATA box Biochemistry Structural Biology Promoter Regions Genetic biology Core promoter-selective transcription activation Biological Sciences TATA Box Physical Sciences RNA polymerase II RNA Polymerase II Transcription factor II D Transcription Transcription factor II A Protein Binding Macromolecular Substances 1.1 Normal biological development and functioning Biophysics macromolecular substances Article Promoter Regions 03 medical and health sciences Genetic Underpinning research Genetics Animals Humans TATA-binding protein Molecular Biology TATA-Binding Protein Associated Factors Nucleic Acid TATA-Box Binding Protein fungi Downstream promoter element Promoter Molecular biology Actins Gene regulation 030104 developmental biology Gene Expression Regulation Transcription Factor TFIID biology.protein Generic health relevance Regulatory Sequences |
| Zdroj: | Biochimica et biophysica acta, vol 1859, iss 4 Xu, M; Gonzalez-Hurtado, E; & Martinez, E. (2016). Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 1859(4), 553-563. doi: 10.1016/j.bbagrm.2016.01.005. UC Riverside: Retrieved from: http://www.escholarship.org/uc/item/7g73z3np |
| ISSN: | 1874-9399 |
| DOI: | 10.1016/j.bbagrm.2016.01.005 |
| Popis: | Gene-specific activation by enhancers involves their communication with the basal RNA polymerase II transcription machinery at the core promoter. Core promoters are diverse and may contain a variety of sequence elements such as the TATA box, the Initiator (INR), and the downstream promoter element (DPE) recognized, respectively, by the TATA-binding protein (TBP) and TBP-associated factors of the TFIID complex. Core promoter elements contribute to the gene selectivity of enhancers, and INR/DPE-specific enhancers and activators have been identified. Here, we identify a TATA box-selective activating sequence upstream of the human β-actin (ACTB) gene that mediates serum response factor (SRF)-induced transcription from TATA-dependent but not INR-dependent promoters and requires the TATA-binding/bending activity of TBP, which is otherwise dispensable for transcription from a TATA-less promoter. The SRF-dependent ACTB sequence is stereospecific on TATA promoters but activates in an orientation-independent manner a composite TATA/INR-containing promoter. More generally, we show that SRF-regulated genes of the actin/cytoskeleton/contractile family tend to have a TATA box. These results suggest distinct TATA-dependent and INR-dependent mechanisms of TFIID-mediated transcription in mammalian cells that are compatible with only certain stereospecific combinations of activators, and that a TBP-TATA binding mechanism is important for SRF activation of the actin/cytoskeleton-related gene family. |
| Databáze: | OpenAIRE |
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