In situ analysis of breast cancer progression in murine models using a macroscopic fluorescence imaging system
| Autor: | Paige J. Baugher, Kristin M. Wall, Suranganie Dharmawardhane, Rebecca Richards-Kortum, Alicia L. Carlson, Michaela R. Hoffmeyer |
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| Rok vydání: | 2006 |
| Předmět: |
In situ
Pathology medicine.medical_specialty Fluorescence-lifetime imaging microscopy Materials science Mice Nude Mice SCID Dermatology Mice Breast cancer Cell Line Tumor medicine Animals Fiber Optic Technology A fibers Optical Fibers Fluorescent Dyes Luminescent Agents Mammary Neoplasms Experimental Cancer medicine.disease Fluorescence Disease Models Animal Luminescent Proteins Spectrometry Fluorescence Tumor progression In situ analysis Disease Progression Female Surgery Biomedical engineering |
| Zdroj: | Lasers in Surgery and Medicine. 38:928-938 |
| ISSN: | 1096-9101 0196-8092 |
| Popis: | Background and Objective: The goal of this study was to use an inexpensive macroscopic imaging system to monitor tumor progression in mouse models in real-time with minimal intervention. Study Design/Materials and Methods: Illumination is provided via a xenon arc lamp and a fiber optic probe which delivers white light or quasi-monochromatic excitation via specific bandpass filters. Fluorescence emission from SCID and nude mice following mammary fat pad injection of red fluorescence protein (RFP)-expressing human breast cancer cell lines was recorded and quantified using a single lens reflex (SLR) digital camera. Results: This simple system enabled the verification of successful tumor take and temporal quantification of tumor progression in mouse models. Conclusion: The macroscopic fluorescence imaging system represents an inexpensive and portable tool to facilitate noninvasive in situ cancer detection with the potential to monitor fluorescent tumor formation and investigation of the efficacy of potential cancer therapeutics. Lasers Surg. Med. 38:928–938, 2006. 2006 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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