Mechanism of action of novel piperazine containing compound toxicant against human liver cancer cells
| Autor: | Kanthimathi, Batoul Sadat Haerian, Sekaran Muniandy, Nima Samie |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cytoskeletal rearrangement
0301 basic medicine lcsh:Medicine Apoptosis Caspase 3 Mitochondrion Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine medicine Cytotoxic T cell MTT assay Piperazine General Neuroscience Cytochrome c lcsh:R Intrinsic apoptosis General Medicine medicine.disease Molecular biology 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis biology.protein General Agricultural and Biological Sciences Liver cancer |
| Zdroj: | PeerJ, Vol 4, p e1588 (2016) |
| ISSN: | 2167-8359 |
| Popis: | The purpose of this study was to assess the cytotoxic potential of a novel piperazine derivative (PCC) against human liver cancer cells. SNU-475 and 423 human liver cancer cell lines were used to determine the IC50 of PCC using the standard MTT assay. PCC displayed a strong suppressive effect on liver cancer cells with an IC50 value of 6.98 ± 0.11 µM and 7.76 ± 0.45 µM against SNU-475 and SNU-423 respectively after 24 h of treatment. Significant dipping in the mitochondrial membrane potential and elevation in the released of cytochrome c from the mitochondria indicated the induction of the intrinsic apoptosis pathway by PCC. Activation of this pathway was further evidenced by significant activation of caspase 3/7 and 9. PCC was also shown to activate the extrinsic pathways of apoptosis via activation of caspase-8 which is linked to the suppression of NF-κB translocation to the nucleus. Cell cycle arrest in the G1 phase was confirmed by flow cytometry and up-regulation of glutathione reductase expression was quantified by qPCR. Results of this study suggest that PCC is a potent anti-cancer agent inducing both intrinsic and extrinsic pathways of apoptosis in liver cancer cell lines. |
| Databáze: | OpenAIRE |
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