Modulation of immune response to Toxoplasma gondii in sheep by immunization with a DNA vaccine encoding ROP1 antigen as a fusion protein with ovine CD154
| Autor: | Elżbieta Hiszczyńska-Sawicka, Lucyna Holec-Gąsior, Józef Kur, Janet Boyu Xu, Richard Sedcole, Hong Li, Roy Bickerstaffe, M. Stankiewicz |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Protozoan Vaccines
Recombinant Fusion Proteins CD40 Ligand Protozoan Proteins Antibodies Protozoan Sheep Diseases Antigens Protozoan Booster dose CHO Cells Injections Intramuscular DNA vaccination Andrology Immune system Cricetulus Antigen Cricetinae parasitic diseases Vaccines DNA Animals CD154 Sheep Domestic CD40 Sheep General Veterinary biology Toxoplasma gondii Membrane Proteins General Medicine biology.organism_classification Toxoplasmosis Animal Immunology biology.protein Parasitology Cattle Female Immunization Intramuscular injection Toxoplasma New Zealand Plasmids |
| Zdroj: | Veterinary parasitology. 183(1-2) |
| ISSN: | 1873-2550 |
| Popis: | CD154 is a cell surface molecule expressed by activated T cells. CD40 and CD154 interaction is critically important in regulating humoral and cell-mediated immune responses. In this study we have investigated whether a DNA vaccine encoding rhoptry protein 1 (ROP1) of Toxoplasma gondii, and encoding ovine CD154 induces an enhanced ROP1-specific immune response in sheep. Two groups of twelve animals received two intramuscular injections, of a DNA plasmid encoding T. gondii ROP1 antigen (group 1) or an ROP1 antigen fused to ovine CD154 (group 2). There were two control groups of sheep. One was injected with an empty vector (group 3) and the other received no injections at all (group 4). The injection of the plasmid containing ROP1 (group 1) at weeks 0 and 4 induced a significant IgG2 response at week 2 which was amplified at week 4 after the booster injection and persisted to week 8 compared to the control animals in groups 3 and 4. For IgG1, significant differences from the control animals were only observed from week 5 onwards. The fusion of CD154 and ROP1 elicited significant IgG1 and IgG2 responses from week 1 which were amplified from weeks 5 to 8 compared to the control animals in groups 3 and 4. The IgG1 response was significantly higher in group 2 animals receiving pROP1-CD154 compared to group 1 receiving pROP1 only. There was no significant difference in IgG2 responses between groups 1 and 2. Significant differences in IFN-γ levels were only observed in treatment group 1 at week 2 and treatment group 2 at weeks 1 and 2 compared to the control animals. The results demonstrated that an intramuscular injection of pROP1-CD154 gene to sheep significantly enhanced their immune response and induced a mixed Th1/Th2 response while the intramuscular injection of pROP1 only induced a Th1-specific immune response. |
| Databáze: | OpenAIRE |
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