Enzymatic Potential of Bacteria and Fungi Isolates from the Sewage Sludge Composting Process
| Autor: | Tatiana Robledo-Mahón, Elisabet Aranda, Concepción Calvo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Thielavia
enzymes 010501 environmental sciences 01 natural sciences lcsh:Technology lcsh:Chemistry 03 medical and health sciences General Materials Science Food science bacteria Sewage sludge Instrumentation lcsh:QH301-705.5 030304 developmental biology 0105 earth and related environmental sciences Fluid Flow and Transfer Processes Mucoromycotina Laccase 0303 health sciences Bjerkandera biology Ascomycota sewage sludge Bacteria Bacillus pumilus Chemistry Lignin-modifying enzyme lcsh:T Process Chemistry and Technology Composting fungi General Engineering Fungi biology.organism_classification lcsh:QC1-999 Computer Science Applications Enzymes lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 biology.protein composting lcsh:Engineering (General). Civil engineering (General) Sludge lcsh:Physics |
| Zdroj: | Applied Sciences Volume 10 Issue 21 Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Applied Sciences, Vol 10, Iss 7763, p 7763 (2020) Digibug. Repositorio Institucional de la Universidad de Granada instname |
| ISSN: | 2017-8433 |
| Popis: | The aim of this study was the isolation and characterisation of the fungi and bacteria during the composting process of sewage sludge under a semipermeable membrane system at full scale, in order to find isolates with enzymatic activities of biotechnological interest. A total of 40 fungi were isolated and enzymatically analysed. Fungal culture showed a predominance of members of Ascomycota and Basidiomycota division and some representatives of Mucoromycotina subdivision. Some noticeable fungi isolated during the mesophilic and thermophilic phase were Aspergillus, Circinella, and Talaromyces. During the maturation phase, some lignin modifying enzyme producers, like Purpureocillium, Thielavia, Bjerkandera, or Dichotomyces, were found. Within this group, Thielavia and Bjerkandera showed high activity with production of laccases and peroxidases. In the bacterial culturome, a total of 128 strains were selected and enzymatically analysed. Bacillales, Actinomycetales, Pseudomonadales, and Lactobacillales were the orders most represented in culture-bacteria. Bacillus pumilus, B. stratosphericus, B. safensis, and Pseudomonas formosensis were the species most e cient in enzyme production, particularly peroxidases, polyphenol oxidases ammonifying activity, and amylases. These results showed that sewage sludge composting piles could represent a source of microorganisms which have adapted to adverse conditions. Junta de Andalucía RNM-7370 CTM2017-84332-R Junta de Andalucía RYC-2013-12481 |
| Databáze: | OpenAIRE |
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