Study on community structure of microbial consortium for the degradation of viscose fiber wastewater
| Autor: | Ding Chaoqun, He Bai, Shiru Jia, Yun-Xia Duan, Hexin Lv, Cheng Zhong, Kun-Rong Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Biotechnology Biomedical Engineering 010501 environmental sciences lcsh:Chemical technology 01 natural sciences Enrichment culture lcsh:Technology Microbiology 03 medical and health sciences lcsh:TP248.13-248.65 lcsh:TP1-1185 Bacillus licheniformis Food science 0105 earth and related environmental sciences biology Renewable Energy Sustainability and the Environment Chemistry lcsh:T Research Pseudomonas DGGE fingerprinting technology Biodegradation Microbial consortium biology.organism_classification Community structure 030104 developmental biology Activated sludge Wastewater Bio-augmentation technology Temperature gradient gel electrophoresis Food Science Biotechnology Viscose fiber wastewater |
| Zdroj: | Bioresources and Bioprocessing, Vol 4, Iss 1, Pp 1-9 (2017) Bioresources and Bioprocessing |
| ISSN: | 2197-4365 |
| Popis: | Background Enrichment culture was applied to obtain microbial consortium from activated sludge samples collected from biodegradation system, a chemical fiber plant in Hebei Province, China. Bacterial composition and community dynamic variation were assessed employing denaturing gradient gel electrophoresis fingerprinting technology based on amplified 16S rRNA genes in the entire process of enrichment culture for viscose fiber wastewater. Results Four bacteria named as VF01, VF02, VF03, and VF04 were isolated from the microbial consortium adopting the spray-plate method. The DNA bands of these four bacteria were corresponded to the predominant DNA bands in the electrophoresis pattern. VF01, VF02, VF03, and VF04 were phylogenetically closed to Bacillus licheniformis, Bacillus subtilis, Paracoccus tibetensis, and Pseudomonas sp. by sequence analysis, respectively. The degradation effects for CODCr of single isolated strain, mixed strains, and microbial consortium (VF) originally screened from viscose fiber wastewater were determined. The degradation ability was as follows: microbial consortium (VF) > mixed strains > single isolated strain. Microbial consortium (VF) showed the optimum degradation rate of CODCr of 87% on 14th day. Degradation of pollutants sped up by bio-augmentation of four strains. The molecular weight distribution of organic matter showed that viscose fiber wastewater contained a certain amount of large molecular organic matter, which could be decomposed into smaller molecular substances by microbial consortium (VF). Conclusions The microbial consortium (VF) obtained from enrichment culture exhibited great potential for CODCr degradation. The screened strains had bio-augmentation functions and the addition of a mixture of four bacteria could speed up the degradation rate of pollutants. |
| Databáze: | OpenAIRE |
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