Morphological and functional characterization of the hemocytes from the pearl oyster Pteria hirundo and their immune responses against Vibrio infections
| Autor: | Augusto Ferrari Hering, Rafael Diego Rosa, Éder C. Schmidt, Marcos Caivano Pedroso de Albuquerque, Jaqueline da Rosa Coelho, Zenilda L. Bouzon, Patricia Mirella da Silva, Graziela Cleuza Vieira, Luciane Maria Perazzolo, Margherita Anna Barracco |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Agglutination Hemocytes Population Aquatic Science Microbiology 03 medical and health sciences Immune system Vibrio Infections Environmental Chemistry Animals education Vibrio Vibrio alginolyticus education.field_of_study Immunity Cellular biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Ostreidae Immunity Innate Immunity Humoral Agglutination (biology) 030104 developmental biology Cytoplasm Immunology 040102 fisheries Ultrastructure 0401 agriculture forestry and fisheries |
| Zdroj: | Fishshellfish immunology. 70 |
| ISSN: | 1095-9947 |
| Popis: | Hemocyte populations of the pearl oyster Pteria hirundo were characterized at morphological, ultrastructural and functional levels. Three main hemocyte populations were identified: hyalinocytes, granulocytes and blast-like cells. Hyalinocytes were the most abundant population (88.2%) characterized by the presence of few or no granules in the cytoplasm and composed by two subpopulations, large and small hyalinocytes. Comparatively, granulocytes represented 2.2% of the hemocyte population and were characterized by the presence of numerous large electron-lucid granules in the cytoplasm. Finally, the blast-like cells (9.5%) were the smallest hemocytes, showing spherical shape and a high nucleus/cytoplasm ratio. Hemocytes exhibited a significant phagocytic capacity for inert particles (38.5%) and showed to be able to produce microbicidal molecules, such as reactive oxygen species (ROS) (ex vivo assays). The immune role of hemocytes was further investigated in the P. hirundo defense against the Gram-negative Vibrio alginolyticus. A significant decrease in the total number of hemocytes was observed at 24 h following injection of V. alginolyticus or sterile seawater (injury control) when compared to naive (unchallenged) animals, indicating the migration of circulating hemocytes to the sites of infection and tissue damage. Bacterial agglutination was only observed against Gram-negative bacteria (Vibrio) but not against to marine Gram-positive-bacteria. Besides, an increase in the agglutination titer was observed against V. alginolyticus only in animals previously infected with this same bacterial strain. These results suggest that agglutinins or lectin-like molecules may have been produced in response to this particular microorganism promoting a specific recognition. The ultrastructural and functional characterization of P. hirundo hemocytes constitutes a new important piece of the molluscan immunity puzzle that can also contribute for the improvement of bivalve production sustainability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect