The effect of medroxyprogesterone on cells of the pituitary pars distalis
| Autor: | H. W. Clapp, Burton L. Baker, Thomas A. Eskin |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Pituitary gland
medicine.medical_specialty Medroxyprogesterone Thyroid Gland Biology Gonadotropic cell General Biochemistry Genetics and Molecular Biology Human chorionic gonadotropin Adrenocorticotropic Hormone Internal medicine Adrenal Glands medicine Endocrine system Animals Thyroid Body Weight Ovary Uterus Organ Size Prolactin Rats Microscopy Electron medicine.anatomical_structure Endocrinology Pituitary Gland Female Rabbits Gonadotropins medicine.drug Hormone |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 140(1) |
| ISSN: | 0037-9727 |
| Popis: | The action of medroxyprogesterone (MAP) on the pituitary par distalis was studied in young female rats receiving doses twice daily ranging from 0.5-1.5 mg/100 g body weight (b.w.) for 14-28 days. Pituitary glands were stained with aledhyde fuchsin and Masson and differential cell counts were done. Immunochemical staining of sections taken from various regions of the gland were made with peroxidase-labeled antibody procedure used with rabbit antisera for rat prolactin human growth hormone porcine corticotropin and human chorionic gonadotropin. Due to questionable specificity of anti-HCG for LH these cells were called gonadotropic cells. MAP had no effect on weight of the body or thyroid glands but reduced the weight of the hypophysis adrenals ovaries and uterus. Treatment of 4 weeks or greater caused marked degranulation and reduction in size of prolactin cells; 0.5 mg/100 g b. w. was as effective as 1.5 mg/100 g b. w. Growth hormone cells were enlarged to a greater extent at the higher dose. Percentage increase of number of growth hormone cells (1.5 mg dose) at 4 weeks compared to controls was significant (p less than 0.01). Effect on gonadotropic cells was less clear: their numbers were reduced in the central and medial areas of the lateral lobes but many were enlarged and more intensly stained than controls. Corticotropic cells were not affected significantly. Conflicting results with earlier studies suggest differences in cells identified as prolactin cells and in the amount of progestational activity exerted by the compounds used. A brief comparison is made to norethynodrel. |
| Databáze: | OpenAIRE |
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