Salmonella enterotoxin (Stn) regulates membrane composition and integrity
| Autor: | Akira Takahashi, Junko Akada, Eiki Yamasaki, Takaaki Shimohata, Akitoyo Ichinose, Joel Moss, Toshiya Hirayama, Kazuyuki Nakamura, Masayuki Nakano, Hisao Kurazono |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Salmonella
Mutant lcsh:Medicine Medicine (miscellaneous) Enterotoxin medicine.disease_cause Virulence factor Western blotting Enterotoxins Mice Immunology and Microbiology (miscellaneous) cell membrane protein CXCL3 chemokine Virulence article immunogold labeling Salmonella enterica U937 Cells Cell biology unclassified drug Protein Transport surgical procedures operative female priority journal wild type Research Article Bacterial Outer Membrane Proteins Protein Binding lcsh:RB1-214 granulocyte macrophage colony stimulating factor monocyte chemotactic protein 3 in vitro study phenotype Molecular Sequence Data animal experiment Neuroscience (miscellaneous) CXCL1 chemokine protein localization Biology General Biochemistry Genetics and Molecular Biology Microbiology in vivo study RANTES proteomics transmission electron microscopy lcsh:Pathology medicine Animals Humans controlled study RNA Messenger human protein interaction protein structure salmonella enterotoxin mouse nonhuman gene deletion bacterial virulence human cell Cell Membrane lcsh:R Wild type Gene Expression Regulation Bacterial Cell Biology Periplasmic space biology.organism_classification bacterial strain Molecular biology outer membrane protein A amino acid sequence Mice Inbred C57BL Protein Subunits CXCL2 chemokine Membrane protein nervous system Genes Bacterial Mutation bacterial membrane Sequence Alignment enterotoxin HeLa Cells |
| Zdroj: | Disease Models & Mechanisms, Vol 5, Iss 4, Pp 515-521 (2012) Disease Models & Mechanisms |
| ISSN: | 1754-8403 |
| Popis: | The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a murine ileal loop model. In these systems, we did not observe differences in virulence phenotypes between wild-type Salmonella and an stn gene-deleted mutant. We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions. The proteomic profiles of the total cell membrane protein fraction differed between wild type and mutant in that there was an absence of a protein in the mutant strain, which was identified as OmpA. By far-western blotting, OmpA was found to interact directly with Stn. To verify this result, the morphology of Salmonella was examined by transmission electron microscopy, with OmpA localization being analyzed by immunogold labeling. Compared with wild-type Salmonella, the mutant strain had a different pole structure and a thin periplasmic space; OmpA was not seen in the mutant. These results indicate that Stn, via regulation of OmpA membrane localization, functions in the maintenance of membrane composition and integrity. Disease Models & Mechanisms, 5(4), pp.515-521; 2012 |
| Databáze: | OpenAIRE |
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