Effect and mechanism of YB-1 knockdown on glioma cell growth, migration, and apoptosis
| Autor: | Huilin Gong, Chenghuan Yu, Jinning Song, Meihe Li, Jin Zheng, Ping Liu, Guanjun Zhang, Shan Gao |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Biophysics Apoptosis Cell Cycle Proteins Biochemistry 03 medical and health sciences 0302 clinical medicine ErbB Cell Movement Glioma Cell Line Tumor medicine Humans RNA Neoplasm Phosphorylation PI3K/AKT/mTOR pathway 030304 developmental biology Cell Proliferation 0303 health sciences Gene knockdown biology Cell growth Chemistry Cyclin-dependent kinase 2 General Medicine Cell cycle medicine.disease Neoplasm Proteins 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research biology.protein Y-Box-Binding Protein 1 Apoptosis Regulatory Proteins Cell Adhesion Molecules |
| Zdroj: | Acta biochimica et biophysica Sinica. 52(2) |
| ISSN: | 1745-7270 |
| Popis: | Y-box binding protein 1 (YB-1) is manifested as its involvement in cell proliferation and differentiation and malignant cell transformation. Overexpression of YB-1 is associated with glioma progression and patient survival. The aim of this study is to investigate the influence of YB-1 knockdown on glioma cell progression and reveal the mechanisms of YB-1 knockdown on glioma cell growth, migration, and apoptosis. It was found that the knockdown of YB-1 decreased the mRNA and protein levels of YB-1 in U251 glioma cells. The knockdown of YB-1 significantly inhibited cell proliferation, colony formation, and migration in vitro and tumor growth in vivo. Proteome and phosphoproteome data revealed that YB-1 is involved in glioma progression through regulating the expression and phosphorylation of major proteins involved in cell cycle, adhesion, and apoptosis. The main regulated proteins included CCNB1, CCNDBP1, CDK2, CDK3, ADGRG1, CDH-2, MMP14, AIFM1, HO-1, and BAX. Furthermore, it was also found that YB-1 knockdown is associated with the hypo-phosphorylation of ErbB, mTOR, HIF-1, cGMP-PKG, and insulin signaling pathways, and proteoglycans in cancer. Our findings indicated that YB-1 plays a key role in glioma progression in multiple ways, including regulating the expression and phosphorylation of major proteins associated with cell cycle, adhesion, and apoptosis. |
| Databáze: | OpenAIRE |
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