Increasing Flt3L availability alters composition of a novel bone marrow lymphoid progenitor compartment
| Autor: | Antonius G. Rolink, Melanie Rauch, Rhodri Ceredig, Gina Balciunaite |
|---|---|
| Přispěvatelé: | Developmental and Molecular Immunology, Department of Clinical and Biological Sciences (DKBW), University of Basel (Unibas), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté]), Saas, Philippe, Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Myelopoiesis
Myeloid Biochemistry MESH: Mice Knockout MESH: Hematopoietic Stem Cells Mice 0302 clinical medicine Leukocytes MESH: Animals MESH: Bone Marrow Transplantation Cells Cultured Bone Marrow Transplantation Mice Knockout Myelopoiesis 0303 health sciences MESH: Antigens CD19 MESH: Dendritic Cells Lymphopoiesis Cell Differentiation Hematology MESH: Lymphopoiesis MESH: Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-kit medicine.anatomical_structure [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH: fms-Like Tyrosine Kinase 3 MESH: Cells Cultured MESH: Cell Differentiation [SDV.IMM] Life Sciences [q-bio]/Immunology Immunology Antigens CD19 Thymus Gland MESH: Antigens CD45 Biology MESH: Leukocytes MESH: Coculture Techniques 03 medical and health sciences medicine Animals Progenitor cell MESH: Mice 030304 developmental biology Cell Biology Dendritic cell Dendritic Cells MESH: Thymus Gland Hematopoietic Stem Cells Coculture Techniques fms-Like Tyrosine Kinase 3 Fms-Like Tyrosine Kinase 3 Cancer research Leukocyte Common Antigens Bone marrow CCL23 MESH: Female 030215 immunology |
| Zdroj: | Blood Blood, American Society of Hematology, 2006, 108 (4), pp.1216-22. ⟨10.1182/blood-2005-10-006643⟩ |
| ISSN: | 0006-4971 1528-0020 |
| Popis: | We have recently described a CD19– B220+CD117low bone marrow subpopulation with B, T, and myeloid developmental potential, which we have called “early progenitors with lymphoid and myeloid potential” or EPLM. These cells also expressed Fms-like tyrosine kinase 3, Flt3, or CD135. Treatment of mice with the corresponding ligand, Flt3L, showed a 50-fold increase in EPLM. In addition to the expected increase in dendritic cell numbers, Flt3L treatment had a reversible inhibitory effect on B lymphopoiesis. Limiting dilution analysis of sorted EPLM from Flt3L-treated mice showed that B-lymphocyte progenitor activity was reduced 20-fold, but that myeloid and T-cell progenitor activity was largely preserved. EPLM from treated mice transiently reconstituted the thymus and bone marrow of recipient mice, generating cohorts of functional T and B cells in peripheral lymphoid organs. Thus, Flt3L treatment results in a dramatic increase in a novel bone marrow cell with lymphoid and myeloid progenitor activity. |
| Databáze: | OpenAIRE |
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