Bio-origami hydrogel scaffolds composed of photocrosslinked PEG bilayers
| Autor: | Tzia Ming Onn, Mustapha Jamal, Thao D. Nguyen, Rohan Fernandes, David H. Gracias, Rui Xiao, Faraz Jivan, Sachin Kadam |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Ultraviolet Rays Biomedical Engineering Pharmaceutical Science Cell Line Polyethylene Glycols Biomaterials chemistry.chemical_compound Mice Tissue engineering Insulin-Secreting Cells PEG ratio medicine Animals Insulin Particle Size Fluorescent Dyes Aqueous solution Tissue Engineering technology industry and agriculture Hydrogels Molecular Weight chemistry Microscopy Fluorescence Self-healing hydrogels Biophysics Self-assembly Swelling medicine.symptom Ethylene glycol Micropatterning Biomedical engineering |
| Zdroj: | Advanced healthcare materials. 2(8) |
| ISSN: | 2192-2659 |
| Popis: | We describe the self-folding of photopatterned poly (ethylene glycol) (PEG)-based hydrogel bilayers into curved and anatomically relevant micrometer-scale geometries. The PEG bilayers consist of two different molecular weights (MWs) and are photocrosslinked en masse using conventional photolithography. Self-folding is driven by differential swelling of the two PEG bilayers in aqueous solutions. We characterize the self-folding of PEG bilayers of varying composition and develop a finite element model which predicts radii of curvature that are in good agreement with empirical results. Since we envision the utility of bio-origami in tissue engineering, we photoencapsulate insulin secreting β-TC-6 cells within PEG bilayers and subsequently self-fold them into cylindrical hydrogels of different radii. Calcein AM staining and ELISA measurements are used to monitor cell proliferation and insulin production respectively, and the results indicate cell viability and robust insulin production for over eight weeks in culture. |
| Databáze: | OpenAIRE |
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