RAPD markörleri kullanılarak Velezia L. (Caryophyllaceae) türlerinin genetik akrabalıklarının belirlenmesi

Autor: POYRAZ, İlham Eröz, SÖZEN, Emel, ATAŞLAR, Ebru, POYRAZ, İsmail
Přispěvatelé: Anadolu Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji Anabilim Dalı, Eröz Poyraz, İlham, Sözen, Emel, Poyraz, İsmail
Rok vydání: 2012
Předmět:
Zdroj: Volume: 36, Issue: 3 293-302
Turkish Journal of Biology
ISSN: 1303-6092
1300-0152
DOI: 10.3906/biy-1012-177
Popis: WOS: 000304071800007
Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high (98.61%) and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identification. Furthermore, it can be efficiently employed in future studies to provide preliminary data for conservation of endangered Velezia species.
Eskisehir Osmangazi University Scientific Research Projects Commission [200319054]
The authors would like to thank Prof Dr Avinoam Danin, Collection Manager of the Hebrew University of Jerusalem, and Hagar Leschner for providing V. fasciculata samples from Palaestina. Support for this study as a project was received from the Eskisehir Osmangazi University Scientific Research Projects Commission in the name of "Taxonomical, Morphological, and Anatomical Investigations on Petrorhagia (Ser.) Link, Velezia L., and Saponaria L. Genera in Turkey" (Project Number 200319054).
Databáze: OpenAIRE
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