Vitamin D alleviates lipopolysaccharide-induced acute lung injury via regulation of the renin-angiotensin system
| Autor: | Jialai Yang, Qiu Zhang, Jian Chen, Hong Zhang, Jun Xu, Qingli Luo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Cancer Research ARDS vitamin D renin-angiotensin system Pharmacology Biochemistry chemistry.chemical_compound 0302 clinical medicine Renin Lung Cells Cultured Evans Blue Angiotensin II lipopolysaccharide Articles respiratory system medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Angiotensin-converting enzyme 2 Molecular Medicine lipids (amino acids peptides and proteins) Angiotensin-Converting Enzyme 2 Bronchoalveolar Lavage Fluid medicine.drug Calcitriol Acute Lung Injury Enzyme-Linked Immunosorbent Assay Peptidyl-Dipeptidase A Lung injury Biology Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 03 medical and health sciences Renin–angiotensin system Genetics medicine Vitamin D and neurology Animals Rats Wistar pulmonary microvascular endothelial cells Molecular Biology Endothelial Cells medicine.disease Rats respiratory tract diseases 030104 developmental biology chemistry Microvessels Immunology |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are the clinical manifestations of severe lung damage and respiratory failure. ALI and ARDS result are associated with high mortality in patients. At present, no effective treatments for ALI and ARDS exist. It is established that vitamin D exhibits anti-inflammatory effects, however, the specific effect of vitamin D on ALI remains largely unknown. The aim of the present study was to investigate whether, and by which mechanism, vitamin D alleviates lipopolysaccharide (LPS)-induced ALI. The results demonstrated that a vitamin D agonist, calcitriol, exhibited a beneficial effect on LPS-induced ALI in rats; calcitriol pretreatment significantly improved LPS-induced lung permeability, as determined using Evans blue dye. Results from reverse transcription-quantitative polymerase chain reaction, western blotting and ELISA analysis demonstrated that calcitriol also modulated the expression of members of the renin-angiotensin system (RAS), including angiotensin (Ang) I-converting enzymes (ACE and ACE2), renin and Ang II, which indicates that calcitriol may exert protective effects on LPS-induced lung injury, at least partially, by regulating the balance between the expression of members of the RAS. The results of the present study may provide novel targets for the future treatment of ALI. |
| Databáze: | OpenAIRE |
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