Proteomic analysis of MON810 and comparable non-GM maize varieties grown in agricultural fields

Autor: Anna Coll, Maria Pla, Michel Rossignol, Pere Puigdomènech, Anna Nadal
Přispěvatelé: Universitat de Girona (UdG), Unité de Recherche Protéomique (PROTEOMIQUE), Institut National de la Recherche Agronomique (INRA), Center for Research in Agricultural Genomics, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Ministerio de Ciencia e Innovación (España)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Proteomics
0106 biological sciences
Agricultural commodity
[SDV]Life Sciences [q-bio]
maize
01 natural sciences
commercial varieties
trasncriptome
wheat
Electrophoresis
Gel
Two-Dimensional
Transgenes
media_common
mass spectrometry
2. Zero hunger
0303 health sciences
Spots
Agriculture
Plants
Genetically Modified
2de
mon810
wild type
substantial equivalence
Biotechnology
Proteomics methods
proteome
gmo
two dimensional gel electrophoresis
Forage
Biology
Zea mays
03 medical and health sciences
Genetic variation
genetically modified organism
Genetics
media_common.cataloged_instance
European Union
European union
marker genes
030304 developmental biology
unexpected effects
Substantial equivalence
business.industry
Genetic Variation
ms
15. Life on land
transgenic maize
Agronomy
gene expression
Animal Science and Zoology
business
Agronomy and Crop Science
010606 plant biology & botany
2 dimensional gel electrophoresis
Zdroj: Transgenic Research
Transgenic Research, Springer Verlag, 2011, 20 (4), pp.939-949. ⟨10.1007/s11248-010-9453-y⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 0962-8819
1573-9368
DOI: 10.1007/s11248-010-9453-y⟩
Popis: Worldwide maize is the second major agricultural commodity and around one-fourth is currently biotech, with significant application of the insect resistant event MON810 particularly in the European Union. Grains are the major commercialized part of the plant, and can be harvested after maturity (for food and feed purposes) or at late milky-starchy stage (for forage uses, with the whole plant). We assessed possible proteomic unintended effects of the MON810 transgene using two-dimensional gel electrophoresis coupled to mass spectrometry. To keep in a realistic scenario we used plants grown in agricultural fields in a region where ~50% of maize was MON810, and analyzed grains at milky-starchy stage. In maize, differential transcripts and metabolites between GM and comparable non-GM varieties tend to be variety specific. Thus, we analyzed two variety pairs, DKC6575/Tietar and PR33P67/PR33P66 which are considered representative of Food and Agriculture Organization 700 and 600 varieties commercially grown in the region. MON810 and non-GM milky-starchy grains had virtually identical proteomic patterns, with a very small number of spots showing fold-variations in the 1-1.8 range. They were all variety specific and had divergent identities and functions. Although 2DE allows the analysis of a limited dataset our results support substantial equivalence between MON810 and comparable non-GM varieties.
This work was financially supported by the Spanish MEC project with ref. AGL2007-65903/AGR. AC received a studentship from the Generalitat de Catalunya (2005FI 00144).
Databáze: OpenAIRE
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