The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova
| Autor: | Didier Y.R. Stainier, Michael Brand, Yutaka Kikuchi, Takamasa Mizoguchi, Gerlinde Reim |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Mesoderm animal structures Embryo Nonmammalian Zygote Ectoderm Biology Histogenesis General Biochemistry Genetics and Molecular Biology Endoderm formation medicine SOXF Transcription Factors Animals Cell Lineage RNA Messenger Promoter Regions Genetic Molecular Biology Zebrafish SOX Transcription Factors Body Patterning Genetics POU domain Stem Cells Endoderm High Mobility Group Proteins Gene Expression Regulation Developmental Embryo Cell Differentiation Cell Biology Zebrafish Proteins biology.organism_classification Cell biology DNA-Binding Proteins medicine.anatomical_structure embryonic structures Female Octamer Transcription Factor-3 Developmental Biology Transcription Factors |
| Zdroj: | Developmental cell. 6(1) |
| ISSN: | 1534-5807 |
| Popis: | The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development. |
| Databáze: | OpenAIRE |
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