Effects of the halothane-sensitivity gene on sarcoplasmic reticulum function
| Autor: | Blake A. Jacobson, Lynn A. Litterer, Charles F. Louis, K. M. Johnson, W. E. Rempel, Esther M. Gallant, James R. Mickelson |
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| Rok vydání: | 1989 |
| Předmět: |
Swine
Physiology Biology Adenosine Triphosphate Species Specificity In vivo Caffeine medicine Animals Receptors Cholinergic Gene Swine Diseases Ryanodine Muscles Endoplasmic reticulum Ryanodine Receptor Calcium Release Channel Cell Biology Molecular biology Phenotype In vitro Cell biology Kinetics Sarcoplasmic Reticulum Genes Calcium Halothane Malignant Hyperthermia Function (biology) medicine.drug |
| Zdroj: | American Journal of Physiology-Cell Physiology. 257:C787-C794 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1989.257.4.c787 |
| Popis: | Pigs heterozygous for the halothane-sensitivity gene exhibit a distinct phenotype with regard to both in vivo and in vitro muscle responses to halothane (E. M. Gallant, J. R. Mickelson, B. D. Roggow, S. K. Donaldson, C. F. Louis, and W. E. Rempel. Am. J. Physiol. 257 (Cell Physiol. 26): C781-C786, 1989). In this paper heavy sarcoplasmic reticulum (SR) preparations were isolated from the muscles of pigs of all three genotypes. The rate of calcium release from SR of pigs homozygous for the halothane-sensitivity gene was approximately twice that of SR from pigs homozygous for the normal allele. Furthermore, in the presence of 6 microM Ca2+, the binding of [3H]ryanodine to SR isolated from the homozygous halothane-sensitive pigs was of a higher affinity than was the binding to SR isolated from the homozygous normal pigs (Kd = 70-90 vs. 265 nM, respectively). The SR from pigs heterozygous for the halothane-sensitivity gene, however, demonstrated intermediate values for the rate of calcium release and the affinity for [3H]ryanodine (Kd = 192 nM). Thus the alterations in heavy SR calcium release and [3H]ryanodine binding in the pigs containing one copy of the halothane-sensitivity gene demonstrate a distinct heterozygote phenotype. These data also suggest that the protein product of this gene is closely associated with, and perhaps identical to, the SR calcium release channel-ryanodine receptor protein. |
| Databáze: | OpenAIRE |
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