Efficient and Selective Enzymatic Acylation Reaction: Separation of Furanosyl and Pyranosyl Nucleosides
| Autor: | V. T. Ravikumar, Sunil K. Singh, Gaurav Shakya, Virinder S. Parmar, Ashok K. Prasad, Jyotirmoy Maity |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Molecular Structure biology Stereochemistry Chemistry Acylation Organic Chemistry Triacylglycerol lipase Glycoside Nucleosides Lipase biology.organism_classification Catalysis Thymine Fungal Proteins chemistry.chemical_compound Biocatalysis Candida antarctica Furans Selectivity Nucleoside Pyrans |
| Zdroj: | The Journal of Organic Chemistry. 73:5629-5632 |
| ISSN: | 1520-6904 0022-3263 |
| DOI: | 10.1021/jo800731u |
| Popis: | Candida antarctica lipase-B (CAL-B) immobilized on lewatite selectively acylated the primary hydroxyl group of the furanosyl nucleoside in a mixture of 1-(alpha-D-arabinofuranosyl)thymine and 1-(alpha-D-arabinopyranosyl)thymine. This selective biocatalytic acylation of furanosyl nucleoside has enabled us an easy separation of arabinofuranosyl thymine from an inseparable mixture with arabinopyranosyl thymine. The primary hydroxyl selective acylation methodology of arabinonucleoside has also been successfully used for the separation of 1-(beta-D-xylofuranosyl)thymine and 1-(beta-D-xylopyranosyl)thymine from a mixture of the two, which demonstrate the generality of the enzymatic methodology for separation of furanosyl and pyranosyl nucleosides. |
| Databáze: | OpenAIRE |
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