Alternative Approaches to Understand Microtubule Cap Morphology and Function
| Autor: | María Ángela Oliva, Federico Gago, Shinji Kamimura, J. Fernando Díaz |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Consejo Superior de Investigaciones Científicas (España), European Commission, Fundación Tatiana Pérez de Guzmán el Bueno, Ministry of Education, Culture, Sports, Science and Technology (Japan), Oliva, María A., Gago, Federico, Kamimura, Shinji, Díaz, José Fernando |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | ACS Omega. 8:3540-3550 |
| ISSN: | 2470-1343 |
| Popis: | 11 p.-3 fig. Microtubules (MTs) are essential cellular machines built from concatenated αβ-tubulin heterodimers. They are responsible for two central and opposite functions from the dynamic point of view: scaffolding (static filaments) and force generation (dynamic MTs). These roles engage multiple physiological processes, including cell shape, polarization, division and movement, and intracellular long-distance transport. At the most basic level, the MT regulation is chemical because GTP binding and hydrolysis have the ability to promote assembly and disassembly in the absence of any other constraint. Due to the stochastic GTP hydrolysis, a chemical gradient from GTP-bound to GDP-bound tubulin is created at the MT growing end (GTP cap), which is translated into a cascade of structural regulatory changes known as MT maturation. This is an area of intense research, and several models have been proposed based on information mostly gathered from macromolecular crystallography and cryo-electron microscopy studies. However, these classical structural biology methods lack temporal resolution and can be complemented, as shown in this mini-review, by other approaches such as time-resolved fiber diffraction and computational modeling. Together with studies on structurally similar tubulins from the prokaryotic world, these inputs can provide novel insights on MT assembly, dynamics, and the GTP cap. This work was supported by Spanish Ministerio de Ciencia e Innovación PID2021-123399OB-I00, PID2019-104545RBI00/AEI/10.13039/501100011033 and and PID2019-104070RB-C22 to M.O.B., J.F.D. and F.G., respectively; Consejo Superior de Investigaciones Científicas PIE 201920E111; the European Commission-NextGenerationsEU (Regulation EU 2020/2094, through CSIC’s Global Health Platform (PTI Salud Global)); and Proyecto de Investigación en Neurociencia Fundación Tatiana Pérez de Guzmán el Bueno 2020 to J.F.D.; and Grant-in-Aid for Exploratory Research (#20657014) and Grants-in-Aid for Scientific Research on Priority Areas (#17H03668, #19K06602) from MEXT, Japan, to S.K |
| Databáze: | OpenAIRE |
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