Reduced Expression of PD-1 in Circulating CD4+ and CD8+ Tregs Is an Early Feature of RRMS
| Autor: | Maja Machcińska, Magdalena Kierasińska, Martyna Michniowska, Marta Maruszewska-Cheruiyot, Ludmiła Szewczak, Rafał Rola, Anna Karlińska, Michael Stear, Katarzyna Donskow-Łysoniewska |
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| Rok vydání: | 2022 |
| Předmět: |
Programmed Cell Death 1 Receptor
Organic Chemistry hemic and immune systems chemical and pharmacologic phenomena General Medicine CD8-Positive T-Lymphocytes T-Lymphocytes Regulatory Catalysis Computer Science Applications Inorganic Chemistry T-Lymphocyte Subsets Transforming Growth Factor beta Humans T cells suppressive markers cytokines relapsing–remitting multiple sclerosis Physical and Theoretical Chemistry Molecular Biology Spectroscopy Uncategorized |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 6; Pages: 3185 |
| DOI: | 10.26181/19703134.v1 |
| Popis: | Altered regulatory T cell (Treg) function could contribute to MS. The expression of activating and inhibitory receptors influences the activity of Tregs. Our aim was to investigate T cell phenotypes in relapsing–remitting MS (RRMS) patients at an early phase of the disease. We examined the influence of demographic parameters on the distribution of CD4+ and CD8+ T cell subclasses by generalized linear modeling. We also studied the expression of the following markers—CTLA-4, GITR, PD-1, FoxP3, Helios, CD28, CD62L, CD103—on T cell subsets from peripheral blood with a 14-color flow cytometry panel. We used an antibody array to define the profiles of 34 Th1/Th2/Th17 cytokines in the serum. Expression of PD-1 and GITR on CD4+ and CD8+ Tregs was decreased in RRMS patients. The proinflammatory factors IFN-γ, IL-17, IL-17F, TGFβ-1, TGFβ-3, IL-1SRII, IL-12 p40, sgp130, IL-6sR were significantly increased in RRMS patients. Therefore, a deficiency of PD-1 and GITR immune checkpoints on CD4+ and CD8+ Tregs is a feature of RRMS and might underlie impaired T cell control. |
| Databáze: | OpenAIRE |
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