Micelles of Progesterone for Topical Eye Administration: Interspecies and Intertissues Differences in Ex Vivo Ocular Permeability
| Autor: | Adrián M. Alambiaga-Caravaca, M.A. Calatayud-Pascual, Vicent Rodilla, Alicia López-Castellano, Carmen Alvarez-Lorenzo, Angel Concheiro |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, UCH. Departamento de Farmacia, Producción Científica UCH 2020 |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
genetic structures
Interspecies ocular permeability differences Pharmaceutical Science lcsh:RS1-441 02 engineering and technology Progesterona - Uso terapéutico progesterone 030226 pharmacology & pharmacy Micelle ocular drug delivery Progesterone - Therapeutic use Article Retinitis pigmentaria - Tratamiento lcsh:Pharmacy and materia medica 03 medical and health sciences interspecies ocular permeability differences 0302 clinical medicine Cornea retinitis pigmentosa medicine Permeability Hen’s egg-chorioallantoic membrane test (HET-CAM) assay Permeabilidad Solubility Progesterone polymeric micelles Soluplus Ocular drug delivery Chemistry solubility Penetration (firestop) Pluronic Poloxamer Retinitis pigmentosa - Treatment 021001 nanoscience & nanotechnology Eye - Diseases - Treatment hen’s egg-chorioallantoic membrane test (HET-CAM) assay eye diseases Sclera Retinitis pigmentosa medicine.anatomical_structure Membrane Ojos - Enfermedades - Tratamiento Biophysics Polymeric micelles sense organs 0210 nano-technology Ex vivo |
| Zdroj: | Pharmaceutics Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname CEU Repositorio Institucional Fundación Universitaria San Pablo CEU (FUSPCEU) Pharmaceutics, Vol 12, Iss 702, p 702 (2020) Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Volume 12 Issue 8 |
| Popis: | Progesterone (PG) may provide protection to the retina during retinitis pigmentosa, but its topical ocular supply is hampered by PG poor aqueous solubility and low ocular bioavailability. The development of efficient topical ocular forms must face up to two relevant challenges: Protective barriers of the eyes and lack of validated ex vivo tests to predict drug permeability. The aims of this study were: (i) To design micelles using Pluronic F68 and Soluplus copolymers to overcome PG solubility and permeability and (ii) to compare drug diffusion through the cornea and sclera of three animal species (rabbit, porcine, and bovine) to investigate interspecies differences. Micelles of Pluronic F68 (3&ndash 4 nm) and Soluplus (52&ndash 59 nm) increased PG solubility by one and two orders of magnitude, respectively and exhibited nearly a 100% encapsulation efficiency. Soluplus systems showed in situ gelling capability in contrast to the low viscosity Pluronic F68 micelles. The formulations successfully passed the hen&rsquo s egg-chorioallantoic membrane test (HET-CAM) test. PG penetration through rabbit cornea and sclera was faster than through porcine or bovine cornea, although the differences were also formulation-dependent. Porcine tissues showed intermediate permeability between rabbit and bovine. Soluplus micelles allowed greater PG accumulation in cornea and sclera whereas Pluronic F68 promoted a faster penetration of lower PG doses. |
| Databáze: | OpenAIRE |
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