Undifferentiated MSCs are able to myelinate DRG neuron processes through p75

Autor: Virginia Rodriguez Menendez, M Ravasi, Giovanni Tredici, S Pasini, Daniele Maggioni, Arianna Scuteri, Mario Bossi
Přispěvatelé: Ravasi, M, Scuteri, A, Pasini, S, Bossi, M, Menendez, V, Maggioni, D, Tredici, G
Rok vydání: 2013
Předmět:
Nerve Tissue Proteins
Receptors
Nerve Growth Factor
Regenerative medicine
Rats
Sprague-Dawley
Myelin
Neurites
medicine
Animals
Receptors
Growth Factor
Cells
Cultured
Myelin Sheath
Neurons
Brain-derived neurotrophic factor
Glial fibrillary acidic protein
biology
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Axons
Coculture Techniques
Rats
Myelin basic protein
Transplantation
Oligodendroglia
Nerve growth factor
medicine.anatomical_structure
nervous system
Immunology
BDNF
Brain Derived Neurotrophic Factor
DRG
DRG neurons
Dorsal Root Ganglia
FUDR
Fluorodeoxyuridine
GFAP
Glial Fibrillary Acidic Protein
MAG
MBP
MSCs
Mesenchymal Stem Cells
Myelin
Myelin Associated Glicoprotein
Myelin Basic Protein
NGF
Nerve Growth Factor
PI
Propidium Iodide
p75
biology.protein
Schwann Cells
Neuroscience
Zdroj: Experimental Cell Research. 319:2989-2999
ISSN: 0014-4827
DOI: 10.1016/j.yexcr.2013.08.016
Popis: Over the last few years the therapeutic approach to demyelinating diseases has radically changed, strategies having been developed aimed at partnering the classic symptomatic treatments with the most advanced regenerative medicine tools. At first, the transplantation of myelinogenic cells, Schwann cells or oligodendrocytes was suggested, but the considerable technical difficulties, (poor availability, difficulties in harvesting and culturing, and the problem of rejection in the event of non-autologous sources), shifted attention towards more versatile cellular types, such as Mesenchymal Stem Cells (MSCs). Recent studies have already demonstrate both in vitro and in vivo that glially-primed MSCs (through exposure to chemical cocktails) have myelogenic abilities. In spite of a large number of papers on glially-differentiated MSCs, little is known about the ability of undifferentiated MSCs to myelinate axons and processes. Here we have demonstrated that also undifferentiated MSCs have the ability to myelinate, since they induce the myelination of rat DRG neuron processes after direct co-culturing. In this process a pivotal role is performed by the p75 receptor. © 2013 Elsevier Inc.
Databáze: OpenAIRE
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