Getting to the Gate: Crystallization of a Sac3CID:Sus1:Cdc31 Complex
| Autor: | Gwendolyn M. Wilmes, Christine Guthrie |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nucleocytoplasmic Transport Proteins
Saccharomyces cerevisiae Proteins PROTEINS Cell Porins Cell Cycle Proteins Saccharomyces cerevisiae Biology Crystallography X-Ray RNA Transport Article law.invention law Transcription (biology) medicine RNA Messenger Crystallization Nuclear pore Gene Molecular Biology Calcium-Binding Proteins Nuclear Proteins RNA-Binding Proteins Cell Biology Cell biology medicine.anatomical_structure Nuclear Pore RNA |
| Zdroj: | Molecular Cell |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2009.03.003 |
| Popis: | Summary The yeast Sac3:Cdc31:Sus1:Thp1 (TREX-2) complex facilitates the repositioning and association of actively transcribing genes with nuclear pores (NPCs)—“gene gating”—that is central to integrating transcription, processing, and mRNA nuclear export. We present here the crystal structure of Sus1 and Cdc31 bound to a central region of Sac3 (the CID domain) that is crucial for its function. Sac3CID forms a long, gently undulating α helix around which one Cdc31 and two Sus1 chains are wrapped. Sus1 has an articulated helical hairpin fold that facilitates its wrapping around Sac3. In vivo studies using engineered mutations that selectively disrupted binding of individual chains to Sac3 indicated that Sus1 and Cdc31 function synergistically to promote NPC association of TREX-2 and mRNA nuclear export. These data indicate Sac3CID provides a scaffold within TREX-2 to integrate interactions between protein complexes to facilitate the coupling of transcription and mRNA export during gene expression. |
| Databáze: | OpenAIRE |
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