Distribution and significance of 14-3-3sigma, a novel myoepithelial marker, in normal, benign, and malignant breast tissue
| Autor: | Dawn Steele, Mario Budroni, Suzanne Parry, Sunil R. Lakhani, Chris Jones, Theodora Gale, Jorge S. Reis-Filho, Antonio Cossu, Peter T. Simpson, Giuseppe Palmieri |
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| Rok vydání: | 2004 |
| Předmět: |
Exonucleases
Pathology medicine.medical_specialty Cytoplasm Stromal cell Mammary gland Breast Neoplasms Biology myoepithelial cells Myoepithelioma Pathology and Forensic Medicine Breast Diseases breast cancer Breast cancer myoepithelial carcinoma medicine Biomarkers Tumor Humans 14-3-3 sigma Proportional Hazards Models Cell Nucleus Tissue microarray Chi-Square Distribution Carcinoma Ductal Breast Myoepithelial cell Anatomical pathology Ductal carcinoma medicine.disease Immunohistochemistry Survival Analysis Neoplasm Proteins Carcinoma Ductal Carcinoma Lobular medicine.anatomical_structure 14-3-3 Proteins Exoribonucleases Female |
| Zdroj: | Journal of pathology 202 (2004): 274–285. doi:10.1002/path.1530 info:cnr-pdr/source/autori:Simpson, PT; Gale, T; Reis, JS; Jones, C; Parry, S; Steele, D; Cossu, A; Budroni, M; Palmieri, G; Lakhani, SR/titolo:Distribution and significance of 14-3-3 sigma, a novel myoepithelial marker, in normal, benign, and malignant breast tissue/doi:10.1002%2Fpath.1530/rivista:Journal of pathology (Print)/anno:2004/pagina_da:274/pagina_a:285/intervallo_pagine:274–285/volume:202 |
| ISSN: | 0022-3417 |
| Popis: | 14-3-3sigma is a candidate tumour suppressor gene transactivated by p53 in response to DNA damage. In gene expression analysis of normal luminal and myoepithelial cells, 14-3-3sigma was preferentially expressed by myoepithelial cells. This study has analysed the immunohistochemical distribution and subcellular localization of 14-3-3sigma in normal breast tissue and in a large series of benign and malignant breast lesions on whole tissue sections and by tissue microarray. Immunohistochemistry demonstrated that 14-3-3sigma was consistently expressed in the cytoplasmic compartment and occasionally in the nuclei of myoepithelial cells arranged as a continuous layer around normal ducts and lobular units, whereas luminal epithelial, stromal, endothelial, pericytic, lipomatous, and neural cells showed no staining. Myoepithelial cells of benign proliferations and pre-invasive lesions were consistently positive for 14-3-3sigma. Strong expression of 14-3-3sigma was evident in one case of ductal carcinoma in situ (5.5%) and in 105/554 invasive cancers (18.9%). Survival data were available for 452 patients with invasive breast carcinoma. 14-3-3sigma cytoplasmic subcellular localization was a statistically significant prognostic factor for the whole series of invasive carcinomas, as well as for those positive for oestrogen (ER) or progesterone receptors (PR). This analysis demonstrates the utility of 14-3-3sigma as a new adjunct antibody for characterization of myoepithelial cells and myoepithelial lesions and it may be a novel prognostic factor for breast cancer patients. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd. |
| Databáze: | OpenAIRE |
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