Improvements of ram semen quality using cactus seed oil during liquid preservation in Tris egg yolk and skim milk based extenders
| Autor: | Larbi Allai, Boubker Nasser, Bouchra El Amiri, Noureddine Louanjli, Jesus Contell, Xavier Druart |
|---|---|
| Přispěvatelé: | Centre Régional de la Recherche Agronomique de Settat, Institut National de la Recherche Agronomique du Maroc (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Biologie Médicale et Biologie de la Reproduction, LABOMAC, Recherche/Development Department, PROISER SL, Laboratoire de Biochimie et Neurosciences [Settat, Maroc], Faculté des Sciences et Techniques [Settat] (FSTS), Université Hassan 1er [Settat]-Université Hassan 1er [Settat], Campus France through two PRAD projects (Programme de Recherche Agronomique pour le Développement), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Tris
endocrine system food.ingredient antioxidant activity Semen DNA fragmentation Biology solvent ram 03 medical and health sciences Semen quality chemistry.chemical_compound 0302 clinical medicine food Food Animals ovin Yolk Botany Skimmed milk sperm parameters stockage au froid rams Food science refrigerated storage reactive oxygen species 030219 obstetrics & reproductive medicine bélier urogenital system diluant 0402 animal and dairy science food and beverages [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm qualité de la semence chemistry Cactus Animal Science and Zoology cactus seed oil motilité du sperme |
| Zdroj: | Small Ruminant Research Small Ruminant Research, Elsevier, 2017, 151, pp.16-21. ⟨10.1016/j.smallrumres.2017.02.001⟩ |
| ISSN: | 0921-4488 |
| DOI: | 10.1016/j.smallrumres.2017.02.001⟩ |
| Popis: | The purpose of this study was to evaluate the effect of addition of cactus seed oil (CSO) on a Tris egg yolk (TEY) and skim milk (SM) extenders, on the sperm quality parameters in ram semen chilled for 72 h. Semen collected from five adult fertile Boujaâd rams was pooled and diluted to a final concentration of 0.8 × 109 spermatozoa/ml in TEY and SM extenders supplemented with CSO at 0, 1, 2, 5, and 10% v/v. Semen was then stored at 5 °C and several sperm parameters (motility, viability, morphology, peroxidation and DNA fragmentation) were assessed after 8, 24, 48 and 72 h of storage. Results showed that the supplementation of TEY and SM extenders with 1% and 2% CSO, respectively contributed to enhance sperm motility and viability and decreased levels of peroxidation and DNA fragmentation during storage. Therefore, we conclude that the optimal concentration of CSO in ram semen extender is 1% and 2%. In addition, our results suggest that CSO in these concentrations can help to maintain ram semen quality during chilled storage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect